Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM | Pseudomonas putida | |
Triton X-100 | 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits 4% aT 1 mM and 33% at 10 mM | Pseudomonas putida | |
EDTA | inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively | Pseudomonas putida | |
Hg2+ | strong inhibition at 1 mM | Pseudomonas putida | |
Li+ | inhibits 17% at 10 mM | Pseudomonas putida | |
Mg2+ | inhibits 19% at 1 mM and 31% at 10 mM | Pseudomonas putida | |
Mn2+ | 21% activation at 1 mM, and 61% inhibition at 10 mM | Pseudomonas putida | |
Ni2+ | inhibits 39% at 1 mM and 63% at 10 mM | Pseudomonas putida | |
SDS | strongly inhibits the enzyme activity at 0.25-0.75% | Pseudomonas putida | |
Triton X-100 | 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity | Pseudomonas putida | |
Zn2+ | strong inhibition at 10 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADP+ | pH 8.5, 30°C | Pseudomonas putida | |
0.018 | - |
NAD+ | pH 8.5, 30°C | Pseudomonas putida | |
0.02 | - |
(R)-mandelate | pH 8.5, 30°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates 44% at 1 mM and 69% at 10 mM | Pseudomonas putida | |
Mn2+ | 21% activation at 1 mM, and 61% inhibition at 10 mM | Pseudomonas putida | |
Na+ | activates 12% at 10 mM | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelate + NAD+ | Pseudomonas putida | - |
phenylglyoxylate + NADH + H+ | - |
r | |
(R)-mandelate + NAD+ | Pseudomonas putida NUST506 | - |
phenylglyoxylate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China) | - |
Pseudomonas putida NUST506 | - |
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China) | - |
Purification (Comment) | Organism |
---|---|
native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
crude enzyme extract, pH 8.5, 30°C | Pseudomonas putida |
0.33 | - |
purified native enzyme, pH 8.5, 30°C | Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelate + NAD+ | - |
Pseudomonas putida | phenylglyoxylate + NADH + H+ | - |
r | |
(R)-mandelate + NAD+ | - |
Pseudomonas putida NUST506 | phenylglyoxylate + NADH + H+ | - |
r | |
(R)-mandelate + NADP+ | - |
Pseudomonas putida | phenylglyoxylate + NADPH + H+ | - |
r | |
(R)-mandelate + NADP+ | - |
Pseudomonas putida NUST506 | phenylglyoxylate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
RMDH | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | activity range, profile overview | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
NADP+ | pH 8.5, 30°C | Pseudomonas putida | |
0.9 | - |
NAD+ | pH 8.5, 30°C | Pseudomonas putida | |
0.9 | - |
(R)-mandelate | pH 8.5, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile overview | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |