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show all sequences of 1.1.1.B65

Characterization of a novel (R)-mandelate dehydrogenase from Pseudomonas putida NUST506

Wang, J.; Feng, J.; Li, W.; Yang, C.; Chen, X.; Bao, B.; Yang, J.; Wang, P.; Li, D.; Shi, R.; J. Mol. Catal. B 120, 23-27 (2015)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
DTT
enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM
Pseudomonas putida
Triton X-100
0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits 4% aT 1 mM and 33% at 10 mM
Pseudomonas putida
EDTA
inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively
Pseudomonas putida
Hg2+
strong inhibition at 1 mM
Pseudomonas putida
Li+
inhibits 17% at 10 mM
Pseudomonas putida
Mg2+
inhibits 19% at 1 mM and 31% at 10 mM
Pseudomonas putida
Mn2+
21% activation at 1 mM, and 61% inhibition at 10 mM
Pseudomonas putida
Ni2+
inhibits 39% at 1 mM and 63% at 10 mM
Pseudomonas putida
SDS
strongly inhibits the enzyme activity at 0.25-0.75%
Pseudomonas putida
Triton X-100
0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
Pseudomonas putida
Zn2+
strong inhibition at 10 mM
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADP+
pH 8.5, 30°C
Pseudomonas putida
0.018
-
NAD+
pH 8.5, 30°C
Pseudomonas putida
0.02
-
(R)-mandelate
pH 8.5, 30°C
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
activates 44% at 1 mM and 69% at 10 mM
Pseudomonas putida
Mn2+
21% activation at 1 mM, and 61% inhibition at 10 mM
Pseudomonas putida
Na+
activates 12% at 10 mM
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-mandelate + NAD+
Pseudomonas putida
-
phenylglyoxylate + NADH + H+
-
-
r
(R)-mandelate + NAD+
Pseudomonas putida NUST506
-
phenylglyoxylate + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
-
Pseudomonas putida NUST506
-
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
-
Purification (Commentary)
Commentary
Organism
native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
crude enzyme extract, pH 8.5, 30°C
Pseudomonas putida
0.33
-
purified native enzyme, pH 8.5, 30°C
Pseudomonas putida
Storage Stability
Storage Stability
Organism
4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-mandelate + NAD+
-
743100
Pseudomonas putida
phenylglyoxylate + NADH + H+
-
-
-
r
(R)-mandelate + NAD+
-
743100
Pseudomonas putida NUST506
phenylglyoxylate + NADH + H+
-
-
-
r
(R)-mandelate + NADP+
-
743100
Pseudomonas putida
phenylglyoxylate + NADPH + H+
-
-
-
r
(R)-mandelate + NADP+
-
743100
Pseudomonas putida NUST506
phenylglyoxylate + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 61000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas putida
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
55
activity range, profile overview
Pseudomonas putida
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
NADP+
pH 8.5, 30°C
Pseudomonas putida
0.9
-
NAD+
pH 8.5, 30°C
Pseudomonas putida
0.9
-
(R)-mandelate
pH 8.5, 30°C
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
activity range, profile overview
Pseudomonas putida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
NADPH
-
Pseudomonas putida
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
DTT
enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM
Pseudomonas putida
Triton X-100
0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
NADPH
-
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits 4% aT 1 mM and 33% at 10 mM
Pseudomonas putida
EDTA
inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively
Pseudomonas putida
Hg2+
strong inhibition at 1 mM
Pseudomonas putida
Li+
inhibits 17% at 10 mM
Pseudomonas putida
Mg2+
inhibits 19% at 1 mM and 31% at 10 mM
Pseudomonas putida
Mn2+
21% activation at 1 mM, and 61% inhibition at 10 mM
Pseudomonas putida
Ni2+
inhibits 39% at 1 mM and 63% at 10 mM
Pseudomonas putida
SDS
strongly inhibits the enzyme activity at 0.25-0.75%
Pseudomonas putida
Triton X-100
0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
Pseudomonas putida
Zn2+
strong inhibition at 10 mM
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADP+
pH 8.5, 30°C
Pseudomonas putida
0.018
-
NAD+
pH 8.5, 30°C
Pseudomonas putida
0.02
-
(R)-mandelate
pH 8.5, 30°C
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
activates 44% at 1 mM and 69% at 10 mM
Pseudomonas putida
Mn2+
21% activation at 1 mM, and 61% inhibition at 10 mM
Pseudomonas putida
Na+
activates 12% at 10 mM
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-mandelate + NAD+
Pseudomonas putida
-
phenylglyoxylate + NADH + H+
-
-
r
(R)-mandelate + NAD+
Pseudomonas putida NUST506
-
phenylglyoxylate + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
crude enzyme extract, pH 8.5, 30°C
Pseudomonas putida
0.33
-
purified native enzyme, pH 8.5, 30°C
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-mandelate + NAD+
-
743100
Pseudomonas putida
phenylglyoxylate + NADH + H+
-
-
-
r
(R)-mandelate + NAD+
-
743100
Pseudomonas putida NUST506
phenylglyoxylate + NADH + H+
-
-
-
r
(R)-mandelate + NADP+
-
743100
Pseudomonas putida
phenylglyoxylate + NADPH + H+
-
-
-
r
(R)-mandelate + NADP+
-
743100
Pseudomonas putida NUST506
phenylglyoxylate + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 61000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas putida
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
55
activity range, profile overview
Pseudomonas putida
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
NADP+
pH 8.5, 30°C
Pseudomonas putida
0.9
-
NAD+
pH 8.5, 30°C
Pseudomonas putida
0.9
-
(R)-mandelate
pH 8.5, 30°C
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
activity range, profile overview
Pseudomonas putida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
Pseudomonas putida
Other publictions for EC 1.1.1.B65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743100
Wang
-
Characterization of a novel ( ...
Pseudomonas putida, Pseudomonas putida NUST506
J. Mol. Catal. B
120
23-27
2015
2
-
-
-
-
-
10
3
-
3
-
2
-
2
-
-
1
-
-
-
2
1
4
1
1
1
1
3
1
1
1
4
-
-
-
2
-
-
4
-
-
-
-
10
-
3
-
3
-
2
-
-
-
1
-
-
2
1
4
1
1
1
1
3
1
1
1
-
-
-
-
-
-
-