BRENDA - Enzyme Database show
show all sequences of 1.1.1.B61

Identification of novel polyphenolic inhibitors of shikimate dehydrogenase (AroE)

Peek, J.; Shi, T.; Christendat, D.; J. Biomol. Screen. 19, 1090-1098 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Arabidopsis thaliana
Crystallization (Commentary)
Crystallization
Organism
crystal structure analysis, PDB ID 2O7S
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
Epicatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epigallocatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
additional information
screening for polyphenolc enzyme inhibitors, overview
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-dehydroshikimate + NADPH
Arabidopsis thaliana
-
shikimate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9SQT8
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydroshikimate + NADPH
-
740771
Arabidopsis thaliana
shikimate + NADP+
-
-
-
r
additional information
The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate
740771
Arabidopsis thaliana
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
assay at
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.002
-
pH 8.8, 25C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
Epicatechin gallate
0.0021
-
pH 8.8, 25C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epigallocatechin gallate
Cloned(Commentary) (protein specific)
Commentary
Organism
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure analysis, PDB ID 2O7S
Arabidopsis thaliana
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.002
-
pH 8.8, 25C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
Epicatechin gallate
0.0021
-
pH 8.8, 25C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epigallocatechin gallate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Epicatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epigallocatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
additional information
screening for polyphenolc enzyme inhibitors, overview
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-dehydroshikimate + NADPH
Arabidopsis thaliana
-
shikimate + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydroshikimate + NADPH
-
740771
Arabidopsis thaliana
shikimate + NADP+
-
-
-
r
additional information
The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate
740771
Arabidopsis thaliana
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
assay at
Arabidopsis thaliana
Other publictions for EC 1.1.1.B61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739956
Peek
The shikimate dehydrogenase fa ...
Arabidopsis thaliana, Populus trichocarpa
Arch. Biochem. Biophys.
566
85-99
2015
-
-
-
1
-
-
-
4
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
2
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
4
2
2
-
-
2
2
-
-
-
-
5
5
-
-
-
740724
Guo
Molecular characterization of ...
Populus trichocarpa, Populus trichocarpa Nisqually-1
J. Biol. Chem.
289
23846-23858
2014
-
-
1
-
-
-
-
3
-
-
-
3
-
2
-
-
1
-
-
5
-
-
5
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
-
-
3
-
-
-
1
-
5
-
-
5
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
740771
Peek
Identification of novel polyph ...
Arabidopsis thaliana
J. Biomol. Screen.
19
1090-1098
2014
-
-
1
1
-
-
3
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
2
-
-
1
2
1
-
-
2
3
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-