BRENDA - Enzyme Database show
show all sequences of 1.1.1.B4

Continuous preparation of (R)-phenyl-ethanediol by catalysis of nanoparticles immobilized bi-enzyme coupling system

Peng, Y.; Zhang, Y.; Chin. J. Proc. Engin. 16, 286-291 (2016)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is used for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (r)-phenylethanediol with in situ NADH regeneration and recycling. Reusable system, method overview
Morganella morganii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-1-phenylethanol + NAD+
Morganella morganii
-
acetophenone + NADH + H+
-
-
?
(R)-1-phenylethanol + NAD+
Morganella morganii CMCC(B)49208
-
acetophenone + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Morganella morganii
-
-
-
Morganella morganii CMCC(B)49208
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
-
742305
Morganella morganii
acetophenone + NADH + H+
-
-
-
?
(R)-1-phenylethanol + NAD+
-
742305
Morganella morganii CMCC(B)49208
acetophenone + NADH + H+
-
-
-
?
additional information
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
742305
Morganella morganii
?
-
-
-
-
additional information
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
742305
Morganella morganii CMCC(B)49208
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
in the coupled nanaparticle-immobilized system
Morganella morganii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in the coupled nanaparticle-immobilized system
Morganella morganii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Morganella morganii
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is used for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (r)-phenylethanediol with in situ NADH regeneration and recycling. Reusable system, method overview
Morganella morganii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Morganella morganii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-1-phenylethanol + NAD+
Morganella morganii
-
acetophenone + NADH + H+
-
-
?
(R)-1-phenylethanol + NAD+
Morganella morganii CMCC(B)49208
-
acetophenone + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
-
742305
Morganella morganii
acetophenone + NADH + H+
-
-
-
?
(R)-1-phenylethanol + NAD+
-
742305
Morganella morganii CMCC(B)49208
acetophenone + NADH + H+
-
-
-
?
additional information
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
742305
Morganella morganii
?
-
-
-
-
additional information
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
742305
Morganella morganii CMCC(B)49208
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
in the coupled nanaparticle-immobilized system
Morganella morganii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in the coupled nanaparticle-immobilized system
Morganella morganii
Other publictions for EC 1.1.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741784
Nealon
I86A/C295A mutant secondary a ...
Thermoanaerobacter ethanolicus
Arch. Biochem. Biophys.
606
151-156
2016
-
-
-
-
3
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
-
17
1
1
-
-
24
1
-
-
2
-
-
-
-
-
-
2
-
3
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
17
1
1
-
-
24
1
-
-
-
-
-
-
-
26
26
742305
Peng
-
Continuous preparation of (R) ...
Morganella morganii, Morganella morganii CMCC(B)49208
Chin. J. Proc. Engin.
16
286-291
2016
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
740394
Chen
Cloning, expression, and chara ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 2343
Enzyme Microb. Technol.
70
18-27
2015
-
-
-
-
-
-
2
3
-
3
-
-
-
2
-
-
-
-
-
-
-
-
13
-
1
-
-
2
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
-
3
-
-
-
-
-
-
-
-
-
-
13
-
1
-
-
2
1
-
-
-
-
-
-
-
2
2
739942
Wang
Cloning, expression, and direc ...
Leifsonia xyli, Leifsonia xyli HS0904
Appl. Microbiol. Biotechnol.
98
8591-8601
2014
-
1
-
-
2
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743703
He
-
Purification and characteriza ...
Kocuria rhizophila
Process Biochem.
49
1107-1112
2014
-
1
-
-
-
-
9
5
-
5
1
-
-
1
-
-
1
-
-
-
1
-
19
1
1
1
3
-
1
1
1
3
-
-
-
-
1
-
3
-
-
-
-
9
-
5
-
5
1
-
-
-
-
1
-
-
1
-
19
1
1
1
3
-
1
1
1
-
-
-
-
-
-
-
721911
Zhou
Biocatalytic characterization ...
Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans DSM 6008
Biotechnol. Lett.
35
359-365
2013
-
1
1
-
-
-
-
1
-
-
1
-
4
2
-
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
739805
Aggarwal
Expression, purification, crys ...
Candida parapsilosis, Candida parapsilosis ATCC 7330
Acta Crystallogr. Sect. F
69
313-315
2013
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723841
Protsko
Crystallization and preliminar ...
Thermoanaerobacter ethanolicus
Acta Crystallogr. Sect. F
66
831-833
2010
-
-
1
1
1
-
-
-
-
-
1
1
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725726
Li
-
Deracemization of aryl seconda ...
Microbacterium oxydans, Microbacterium oxydans ECU2010
J. Mol. Catal. B
64
48-52
2010
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces cerevisiae, Saccharomyces pastorianus, Saccharomyces pastorianus Y379-50
Chem. Biol. Interact.
178
16-23
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697839
Pennacchio
Role of Tryptophan 95 in subst ...
Saccharolobus solfataricus
Extremophiles
13
751-761
2009
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
711860
Machielsen
-
Cofactor engineering of Lactob ...
Lactobacillus brevis
Eng. Life Sci.
9
38-44
2009
-
-
1
-
5
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
4
4
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
-
-
-
7
5
-
5
4
-
1
1
-
-
1
-
-
-
-
-
7
1
2
1
4
4
2
2
-
3
-
-
-
1
1
1
3
-
-
-
-
7
-
5
-
5
4
-
1
-
-
1
-
-
-
-
7
1
2
1
4
4
2
2
-
-
-
-
-
-
-
-
688747
Nie
A novel NADH-dependent carbony ...
Candida parapsilosis
Lett. Appl. Microbiol.
44
555-562
2007
-
-
-
-
-
-
9
-
1
1
2
-
-
1
-
-
1
-
-
-
1
1
10
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
-
1
1
2
-
-
-
-
1
-
-
1
1
10
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687864
Isobe
Thermostable NAD+-dependent (R ...
Burkholderia sp., Burkholderia sp. AIU 652
J. Biosci. Bioeng.
96
387-393
2003
-
-
-
-
-
-
6
15
-
-
2
-
-
3
-
-
1
-
-
-
1
-
24
1
1
-
1
-
3
-
-
3
-
1
-
-
-
-
3
-
-
-
-
6
-
15
-
-
2
-
-
-
-
1
-
-
1
-
24
1
1
-
1
-
3
-
-
1
-
-
-
-
-
-
688571
Bradshaw
-
A Pseudomonas sp. alcohol dehy ...
Pseudomonas sp., Pseudomonas sp. PED
J. Org. Chem.
57
1526-1532
1992
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
1
-
24
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
24
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-