Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43 cells | Thermoanaerobacter ethanolicus |
Protein Variants | Comment | Organism |
---|---|---|
I86A | the mutant shows reversed stereoselectivity (R) compared to the wild type enzyme | Thermoanaerobacter ethanolicus |
W110A | the mutant shows imperfect wild type stereoselectivity (S) | Thermoanaerobacter ethanolicus |
W110A/I86A | the mutant enzyme is completely non-stereoselective for the reduction of 2-hexanone.Additionally, an extremely low level of stereoselectivity (i.e. from 10% to 24%) is observed for the reduction of aryl-aliphatic ketones and aliphatic ketones | Thermoanaerobacter ethanolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin column chromatography | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenyl 2-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | phenylacetone + NADPH + H+ | - |
? | |
1-(3-fluorophenyl)ethan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(3-fluorophenyl)ethan-1-one + NADPH + H+ | - |
? | |
1-(3-methoxyphenyl)propan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(3-methoxyphenyl)propan-2-one + NADPH + H+ | - |
? | |
1-(3-methylphenyl)ethan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(3-methylphenyl)ethan-1-one + NADPH + H+ | - |
? | |
1-(4-fluorophenyl)ethan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(4-fluorophenyl)ethan-1-one + NADPH + H+ | - |
? | |
1-(4-fluorophenyl)propan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(4-fluorophenyl)propan-2-one + NADPH + H+ | - |
? | |
1-(4-methylphenyl)ethan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(4-methylphenyl)ethan-1-one + NADPH + H+ | - |
? | |
1-(4-methylphenyl)propan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-(4-methylphenyl)propan-2-one + NADPH + H+ | - |
? | |
1-phenoxypropan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-phenoxypropan-2-one + NADPH + H+ | - |
? | |
1-phenylbutan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-phenylbutan-2-one + NADPH + H+ | - |
? | |
1-phenylpropan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 1-phenylpropan-2-one + NADPH + H+ | - |
? | |
4-methylpentan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 4-methylpentan-2-one + NADPH + H+ | - |
? | |
4-phenylbutan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | 4-phenylbutan-2-one + NADPH + H+ | - |
? | |
heptan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | heptanal + NADPH + H+ | - |
? | |
heptan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | heptan-2-one + NADPH + H+ | - |
? | |
hexan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | hexanal + NADPH + H+ | - |
? | |
hexan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | hexan-2-one + NADPH + H+ | - |
? | |
octan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | octanal + NADPH + H+ | - |
? | |
octan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | octan-2-one + NADPH + H+ | - |
? | |
pentan-1-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | pentanal + NADPH + H+ | - |
? | |
pentan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | pentan-2-one + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S-ADH | - |
Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermoanaerobacter ethanolicus |