Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 11% inhibition | Escherichia coli | |
Cu2+ | 5 mM, 88% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | 37°C, pH 7.4 | Escherichia coli | |
0.84 | - |
NADH | 37°C, pH 7.4 | Escherichia coli | |
0.89 | - |
NADPH | 37°C, pH 7.4 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Fcf1 is a divalent cation-independent enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40700 | - |
x * 40700, His6-tagged fusion protein, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-fucopyranose + NADP+ | Escherichia coli | the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADP+ | Escherichia coli O52 | the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli O52 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-tagged fusion protein | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, in 50% glycerol, stable for at least 5 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-fucopyranose + NADH + H+ | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADH + H+ | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli O52 | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADP+ | the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway | Escherichia coli | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADP+ | the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway | Escherichia coli O52 | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADPH + H+ | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+ | - |
? | |
dTDP-alpha-D-fucopyranose + NADPH + H+ | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli O52 | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40700, His6-tagged fusion protein, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase | - |
Escherichia coli |
Fcf1 | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 37 | - |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | 4°C: 47% of maximal activity, 50°C: 36% of maximal activity | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.78 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | 37°C, pH 7.4 | Escherichia coli | |
6.76 | - |
NADPH | 37°C, pH 7.4 | Escherichia coli | |
26.67 | - |
NADH | 37°C, pH 7.4 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli | |
NADPH | NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52 | Escherichia coli |
physiological function | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | 37°C, pH 7.4 | Escherichia coli | |
7.6 | - |
NADPH | 37°C, pH 7.4 | Escherichia coli | |
31.75 | - |
NADH | 37°C, pH 7.4 | Escherichia coli |