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Literature summary for 1.1.1.B20 extracted from

  • Nielsen, D.R.; Yoon, S.H.; Yuan, C.J.; Prather, K.L.
    Metabolic engineering of acetoin and meso-2, 3-butanediol biosynthesis in E. coli (2010), Biotechnol. J., 5, 274-284.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bdh1, phylogenetic analysis, expression in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC? expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively, disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion Saccharomyces cerevisiae
phylogenetic analysis, enzyme expression in Escherichia coli strains MG1655(DE3) and YYC202(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information expression of gene bdh1 from Saccharomyces cervisiae in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC-/- expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively. Disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion. Optimization of 2,3-butanediol production in Escherichia coli, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-acetoin + NADH + H+ Saccharomyces cerevisiae
-
meso-2,3-butanediol + NAD+
-
r
(3R)-acetoin + NADH + H+ Escherichia coli low activity in vivo meso-2,3-butanediol + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
gene bdh1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-acetoin + NADH + H+
-
Escherichia coli meso-2,3-butanediol + NAD+
-
r
(3R)-acetoin + NADH + H+
-
Saccharomyces cerevisiae meso-2,3-butanediol + NAD+
-
r
(3R)-acetoin + NADH + H+ low activity in vivo Escherichia coli meso-2,3-butanediol + NAD+
-
r

Synonyms

Synonyms Comment Organism
2,3-BD dehydrogenase
-
Escherichia coli
2,3-BD dehydrogenase
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli
NADH
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
evolution phylogenetic analysis Escherichia coli
evolution phylogenetic analysis Saccharomyces cerevisiae