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Literature summary for 1.1.1.97 extracted from

  • Scott, R.E.; Lam, K.S.; Gaucher, G.M.
    Stabilization and purification of the secondary metabolism specific enzyme, m-hydroxybenzylalcohol dehydrogenase (1986), Can. J. Microbiol., 32, 167-175.
    View publication on PubMed
Search for more literature for 1.1.1.97

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1.0 mM and 10 mM, 56% increase in activity, enzyme activity in cell extracts Penicillium urticae

General Stability

General Stability Organism
14 min half-life of crude enzyme at 30°C in 100 mM Tris-HCl, pH 7.6, half-life is increased to 77 and 80 min by 2 mM MnCl2 and FeCl3 respectively, 3 mM NADPH increase half-life to 146 min Penicillium urticae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Penicillium urticae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzyl alcohol + NADP+ Penicillium urticae
-
 3-hydroxybenzaldehyde + NADPH
-
 r

Organism

Organism UniProt Comment Textmining
Penicillium urticae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Bio-Gel A, 2',5'-ADP-agarose, DEAE-Sephacell, 2',5'-ADP-agarose Penicillium urticae

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
522
-
-
 Penicillium urticae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzyl alcohol + NADP+
-
 Penicillium urticae 3-hydroxybenzaldehyde + NADPH
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Penicillium urticae