Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-alanine | activates | Marchantia polymorpha | |
L-homocysteine | - |
Marchantia polymorpha | |
L-homoserine | - |
Marchantia polymorpha | |
L-methionine | - |
Marchantia polymorpha | |
L-valine | - |
Marchantia polymorpha |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Marchantia polymorpha |
General Stability | Organism |
---|---|
activity of the recombinant enzyme (MtPGDH) is almost lost within 24 h after purification when it is stored in 20 mM phosphate buffer, but not in 100 mM phosphate buffer or in 20 mM phosphate buffer containing 100 mM sodium chloride or potassium chloride | Marchantia polymorpha |
phosphate ions stabilize the enzyme | Marchantia polymorpha |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-serine | 40% inhibition in a cooperative manner | Marchantia polymorpha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
NAD+ | pH 9.0, temperature not specified in the publication | Marchantia polymorpha | |
0.49 | - |
3-phospho-D-glycerate | pH 9.0, temperature not specified in the publication | Marchantia polymorpha |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme contains a putative transit peptide for chloroplast localization | Marchantia polymorpha | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Marchantia polymorpha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Marchantia polymorpha | - |
3-phosphooxypyruvate + NADH + H+ | - |
r | |
3-phosphooxypyruvate + NADH + H+ | Marchantia polymorpha | - |
3-phospho-D-glycerate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marchantia polymorpha | A0A2R6X868 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Marchantia polymorpha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
antheridiophore | - |
Marchantia polymorpha | - |
archegoniophore | - |
Marchantia polymorpha | - |
sporeling | - |
Marchantia polymorpha | - |
sporophyte | - |
Marchantia polymorpha | - |
thallus | - |
Marchantia polymorpha | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.65 | - |
pH 9.0, temperature not specified in the publication | Marchantia polymorpha |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Marchantia polymorpha | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
3-phosphooxypyruvate + NADH + H+ | - |
Marchantia polymorpha | 3-phospho-D-glycerate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60000, SDS-PAGE | Marchantia polymorpha |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate dehydrogenase | - |
Marchantia polymorpha |
MARPO_0030s0029 | - |
Marchantia polymorpha |
PGDH | - |
Marchantia polymorpha |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
NAD+ | pH 9.0, temperature not specified in the publication | Marchantia polymorpha | |
25 | - |
3-phospho-D-glycerate | pH 9.0, temperature not specified in the publication | Marchantia polymorpha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Marchantia polymorpha |
General Information | Comment | Organism |
---|---|---|
metabolism | first committed enzyme of the phosphorylated pathway of L-serine biosynthesis. Regulated by negative feedback from L-serine in bacteria | Marchantia polymorpha |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
3-phospho-D-glycerate | pH 9.0, temperature not specified in the publication | Marchantia polymorpha | |
260 | - |
NAD+ | pH 9.0, temperature not specified in the publication | Marchantia polymorpha |