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Literature summary for 1.1.1.95 extracted from

  • Akashi, H.; Okamura, E.; Nishihama, R.; Kohchi, T.; Hirai, M.Y.
    Identification and biochemical characterization of the serine biosynthetic enzyme 3-phosphoglycerate dehydrogenase in Marchantia polymorpha (2018), Front. Plant Sci., 9, 956 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
L-alanine activates Marchantia polymorpha
L-homocysteine
-
Marchantia polymorpha
L-homoserine
-
Marchantia polymorpha
L-methionine
-
Marchantia polymorpha
L-valine
-
Marchantia polymorpha

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Marchantia polymorpha

General Stability

General Stability Organism
activity of the recombinant enzyme (MtPGDH) is almost lost within 24 h after purification when it is stored in 20 mM phosphate buffer, but not in 100 mM phosphate buffer or in 20 mM phosphate buffer containing 100 mM sodium chloride or potassium chloride Marchantia polymorpha
phosphate ions stabilize the enzyme Marchantia polymorpha

Inhibitors

Inhibitors Comment Organism Structure
L-serine 40% inhibition in a cooperative manner Marchantia polymorpha

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.096
-
NAD+ pH 9.0, temperature not specified in the publication Marchantia polymorpha
0.49
-
3-phospho-D-glycerate pH 9.0, temperature not specified in the publication Marchantia polymorpha

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme contains a putative transit peptide for chloroplast localization Marchantia polymorpha 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Marchantia polymorpha

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glycerate + NAD+ Marchantia polymorpha
-
3-phosphooxypyruvate + NADH + H+
-
r
3-phosphooxypyruvate + NADH + H+ Marchantia polymorpha
-
3-phospho-D-glycerate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Marchantia polymorpha A0A2R6X868
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Marchantia polymorpha

Source Tissue

Source Tissue Comment Organism Textmining
antheridiophore
-
Marchantia polymorpha
-
archegoniophore
-
Marchantia polymorpha
-
sporeling
-
Marchantia polymorpha
-
sporophyte
-
Marchantia polymorpha
-
thallus
-
Marchantia polymorpha
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.65
-
pH 9.0, temperature not specified in the publication Marchantia polymorpha

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NAD+
-
Marchantia polymorpha 3-phosphooxypyruvate + NADH + H+
-
r
3-phosphooxypyruvate + NADH + H+
-
Marchantia polymorpha 3-phospho-D-glycerate + NAD+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 60000, SDS-PAGE Marchantia polymorpha

Synonyms

Synonyms Comment Organism
3-phosphoglycerate dehydrogenase
-
Marchantia polymorpha
MARPO_0030s0029
-
Marchantia polymorpha
PGDH
-
Marchantia polymorpha

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
NAD+ pH 9.0, temperature not specified in the publication Marchantia polymorpha
25
-
3-phospho-D-glycerate pH 9.0, temperature not specified in the publication Marchantia polymorpha

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Marchantia polymorpha

General Information

General Information Comment Organism
metabolism first committed enzyme of the phosphorylated pathway of L-serine biosynthesis. Regulated by negative feedback from L-serine in bacteria Marchantia polymorpha

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
51
-
3-phospho-D-glycerate pH 9.0, temperature not specified in the publication Marchantia polymorpha
260
-
NAD+ pH 9.0, temperature not specified in the publication Marchantia polymorpha