Cloned (Comment) | Organism |
---|---|
expression as selenomethionine-labeled enzyme in strain B834 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme, in presence of 2 mM NAD+ and 5 mM 2-ketoglutarate, euqilibration against precipitation solution containing 1.18-1.25 M ammonium sulfate and 100 mM potassium phosphate, pH 6.4, 18°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.24 A resolution | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Escherichia coli | first step in biosynthesis of L-serine, Vmax regulation through domain and subunit changes, overview | 3-phosphohydroxypyruvate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9T0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant selenomethionine-labeled enzyme from strain B834 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + H+ | - |
Escherichia coli | 2-hydroxyglutarate + NAD+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r | |
3-phospho-D-glycerate + NAD+ | first step in biosynthesis of L-serine, Vmax regulation through domain and subunit changes, overview | Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | quarternary structure of the active enzyme, conformational changes through domain-domain reorientation, stereochemical model, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PGDH | - |
Escherichia coli |
phosphoglycerate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |