Application | Comment | Organism |
---|---|---|
biotechnology | metabolic engineering of Corynebacterium glutamicum for L-serine production by enzyme overexpression | Corynebacterium glutamicum |
synthesis | metabolic engineering of Corynebacterium glutamicum for L-serine production by enzyme overexpression | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
gene serA, overexpression in Escherichia coli strains DH5alphaMCR and GM2929, co-overexpression of serA with other enzymes of the L-serine biosynthesis pathway leads to increased L-serine production in Corynebacterium glutamicum mutant serADELAT197 | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an L-serine-insensitive mutant serADELAT197 | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Corynebacterium glutamicum | |
L-serine | feedback regulation of the wild-type enzyme | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Corynebacterium glutamicum | first step in biosynthesis of L-serine, pathway regulation, overview | 3-phosphohydroxypyruvate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene serA, ATCC 13032 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Corynebacterium glutamicum | 3-phosphohydroxypyruvate + NADH | - |
? | |
3-phospho-D-glycerate + NAD+ | first step in biosynthesis of L-serine, pathway regulation, overview | Corynebacterium glutamicum | 3-phosphohydroxypyruvate + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum |