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Literature summary for 1.1.1.95 extracted from
- 3-Phosphoglycerate dehydrogenase from seedlings of Pisum sativum (1975), Methods Enzymol., 41, 278-281.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-methionine | maximum activation at 10 mM | Pisum sativum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-serine | - |
Pisum sativum | |
| p-hydroxymercuribenzoate | - |
Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADH | - |
Pisum sativum | |
| 0.05 | - |
3-phospho-D-glycerate | - |
Pisum sativum | |
| 0.125 | - |
NAD+ | - |
Pisum sativum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphoglycerate + NAD+ | Pisum sativum | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | 3-phosphohydroxypyruvate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.8 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, 40% loss of activity, 7 days | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | - |
Pisum sativum | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phosphoglycerate + NAD+ | - |
Pisum sativum | 3-phosphohydroxypyruvate + NADH | - |
r | |
| 3-phosphoglycerate + NAD+ | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | Pisum sativum | 3-phosphohydroxypyruvate + NADH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
NADH oxidation | Pisum sativum |
| 9.5 | - |
oxidation of D-3-phosphoglycerate | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pisum sativum | |
| NADH | A-stereospecific for NADH | Pisum sativum | |
| NADP+ | ineffective | Pisum sativum | |
| NADPH | 25% of activity with NADH, can not replace NADH in standard assay concentration | Pisum sativum | |
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Release 2023.1 (January 2023)
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