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Literature summary for 1.1.1.94 extracted from

  • Kito, M.; Pizer, L.I.
    Purification and regulatory properties of the biosynthetic L-glycerol 3-phosphate dehydrogenase from Escherichia coli (1969), J. Biol. Chem., 244, 3316-3323.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 100 mM, 68% inhibition, 10 mM, 8% inhibition Escherichia coli
DL-glycerol 3-phosphate 0.05 mM, 26% inhibition Escherichia coli
glycerol 3-phosphate 50% inhibition with 0.035 mM, sigmoid inhibition curve, competitive inhibition vs. glycerone phosphate, uncompetitive vs. NADPH Escherichia coli
K2PO4- 100 mM, 95% inhibition, 10 mM, 27% inhibition Escherichia coli
K2SO4 100 mM, 60% inhibition Escherichia coli
KCl 100 mM, 47% inhibition Escherichia coli
Na2PO4- 100 mM, 92% inhibition, 10 mM, 27% inhibition Escherichia coli
Na2SO4 100 mM, 63% inhibition Escherichia coli
NaCl 100 mM, 47% inhibition Escherichia coli
NH4Cl 100 mM, 37% inhibition Escherichia coli
palmitoyl-CoA 0.0015 mM, 52% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH
-
Escherichia coli
0.17
-
glycerone phosphate
-
Escherichia coli
0.21
-
glycerol-3-phosphate
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD(P)+ Escherichia coli
-
glycerone phosphate + NAD(P)H + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-150, DEAE-Sephadex Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4800
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD(P)+
-
Escherichia coli glycerone phosphate + NAD(P)H + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
deamino-NAD+ 2fold activity compared to NAD+ Escherichia coli
NAD+
-
Escherichia coli
NADP+ 2fold activity compared to NAD+ Escherichia coli