Application | Comment | Organism |
---|---|---|
analysis | quantification of L-tartrate in wine by a stopped-flow injection system with an immobilized enzyme reactor and fluorescence detection, the enzyme is immobilized on aminopropyl-controlled pore glass beads with glutaraldehyde | Escherichia coli |
General Stability | Organism |
---|---|
the enzyme reactor is stored at 5°C in 0.1 M phosphate buffer, pH 8.0, 10 mM dithiothreitol, when not in use, 40% loss of activity after 1 week, then activity maintains a constant value for 1 month | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | optimal concentration: 50 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tartrate + NAD+ | - |
Escherichia coli | oxaloglycolate + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |