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Literature summary for 1.1.1.91 extracted from
- Deciphering the origin, evolution, and physiological function of the subtelomeric aryl-alcohol dehydrogenase gene family in the yeast Saccharomyces cerevisiae (2018), Appl. Environ. Microbiol., 84, e01553-17 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AAD1, phylogenetic analysis, subcloning of tagged PcAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, activity in BY4741 cell crude extracts expressing PcAad1p is 2fold higher than that of the blank-plasmid control | Phanerodontia chrysosporium |
| gene AAD14, phylogenetic analysis, subcloning of tagged ScAAD14 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd14p does not significantly differ from control | Saccharomyces cerevisiae |
| gene AAD4, phylogenetic analysis, subcloning of tagged ScAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd4p does not significantly differ from control | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a 46-residue N-terminal truncation mutant | Saccharomyces cerevisiae |
| Y76C | site-directed mutagenesis | Phanerodontia chrysosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium |  |
| 1.7 | - |
benzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 3.4 | - |
cinnamaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
| Saccharomyces cerevisiae | P42884 | - |
- |
| Saccharomyces cerevisiae | Q07747 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | P42884 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | Q07747 | - |
- |
| Saccharomyces cerevisiae BY4741 | P42884 | - |
- |
| Saccharomyces cerevisiae BY4741 | Q07747 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dimethoxybenzaldehyde + NADPH + H+ | - |
Phanerodontia chrysosporium | 3,4-dimethoxybenzol + NADP+ | - |
r | |
| 3,4-dimethoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 3,4-dimethoxybenzol + NADP+ | - |
r | |
| 3,4-dimethoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae BY4741 | 3,4-dimethoxybenzol + NADP+ | - |
r | |
| 3,4-dimethoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | 3,4-dimethoxybenzol + NADP+ | - |
r | |
| 4-nitrobenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 4-nitrobenzylalcohol + NADP+ | - |
r | |
| 4-nitrobenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae BY4741 | 4-nitrobenzylalcohol + NADP+ | - |
r | |
| 4-nitrobenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | 4-nitrobenzylalcohol + NADP+ | - |
r | |
| benzaldehyde + NADPH + H+ | - |
Phanerodontia chrysosporium | benzyl alcohol + NADP+ | - |
r | |
| cinnamaldehyde + NADPH + H+ | - |
Phanerodontia chrysosporium | cinnamoyl alcohol + NADP+ | - |
r | |
| heptanal + NADPH + H+ | - |
Phanerodontia chrysosporium | heptanol + NADP+ | - |
r | |
| heptanal + NADPH + H+ | - |
Saccharomyces cerevisiae | heptanol + NADP+ | - |
r | |
| heptanal + NADPH + H+ | - |
Saccharomyces cerevisiae BY4741 | heptanol + NADP+ | - |
r | |
| heptanal + NADPH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | heptanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | - |
Phanerodontia chrysosporium | hexanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae | hexanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae BY4741 | hexanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | hexanol + NADP+ | - |
r | |
| additional information | substrate specificity, overview. PcAad1p is active on a broader spectrum of aryl-aldehyde substrates. PcAad1p can also act as an oxidase against several aliphatic and aromatic alcohols (at pH 10.3, with NADP+ as a cofactor) | Phanerodontia chrysosporium | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde | Saccharomyces cerevisiae BY4741 | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde | Saccharomyces cerevisiae BY4741 | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
| additional information | substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAD1 | - |
Phanerodontia chrysosporium |
| AAD14 | - |
Saccharomyces cerevisiae |
| Aad14p | - |
Saccharomyces cerevisiae |
| Aad1p | - |
Phanerodontia chrysosporium |
| AAD4 | - |
Saccharomyces cerevisiae |
| Aad4p | - |
Saccharomyces cerevisiae |
| aryl-alcohol dehydrogenase | - |
Phanerodontia chrysosporium |
| aryl-alcohol dehydrogenase | - |
Saccharomyces cerevisiae |
| PcAad1p | - |
Phanerodontia chrysosporium |
| ScAad14p protein | - |
Saccharomyces cerevisiae |
| ScAad4p protein | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
4-nitrobenzaldehyde | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.77 | - |
Heptanal | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.83 | - |
hexanal | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 1.07 | - |
Heptanal | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 2.3 | - |
Heptanal | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 2.37 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 2.87 | - |
hexanal | pH 6.1, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 4.12 | - |
hexanal | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 7.17 | - |
benzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 8.83 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 11.17 | - |
cinnamaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
assay at | Phanerodontia chrysosporium |
| 6.1 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADH | Saccharomyces cerevisiae | |
| additional information | the enzyme is active with NADPH and NADH | Phanerodontia chrysosporium | |
| NADH | - |
Phanerodontia chrysosporium | |
| NADP+ | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
| NADPH | preferred cofactor | Phanerodontia chrysosporium | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | yeast AAD gene expression is not induced by aryl-aldehydes | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analysis | Phanerodontia chrysosporium |
| evolution | function and phylogeny of the yeast Aad gene family reveals that only two genes of the family (AAD4 and AAD14) encode enzymatic activities on aliphatic and aryl-aldehydes, whereas the other five putative members are being pseudogenized | Saccharomyces cerevisiae |
| evolution | natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. Phylogenetic analysis | Saccharomyces cerevisiae |
| malfunction | natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. No mutations in Asd14 detected. Yeast AAD overexpression does not increase yeast resistance to aryl-aldehyde compounds | Saccharomyces cerevisiae |
| malfunction | natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. Yeast AAD overexpression does not increase yeast resistance to aryl-aldehyde compounds | Saccharomyces cerevisiae |
| physiological function | Phanerochaete chrysosporium Aad1p can reduce aryl-aldehyde derivatives to their corresponding less-toxic alcohol forms | Phanerodontia chrysosporium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.29 | - |
cinnamaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 4.2 | - |
benzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 735.8 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, temperature not specified in the publication | Phanerodontia chrysosporium | |
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