Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 125% activity at 20% (v/v) acetone | Yokenella sp. WZY002 | |
dithiothreitol | 123% activity at 1 mM | Yokenella sp. WZY002 | |
EDTA | 110% activity at 1 mM | Yokenella sp. WZY002 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Yokenella sp. WZY002 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Yokenella sp. WZY002 | |
Al3+ | 13.5% inhibition at 1 mM | Yokenella sp. WZY002 | |
Co2+ | 80.5% inhibition at 1 mM | Yokenella sp. WZY002 | |
Cu2+ | complete inhibition at 1 mM | Yokenella sp. WZY002 | |
Fe2+ | 39.7% inhibition at 1 mM | Yokenella sp. WZY002 | |
iodoacetate | 94.5% residual activity at 10 mM | Yokenella sp. WZY002 | |
Zn2+ | 99.5% inhibition at 1 mM | Yokenella sp. WZY002 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
benzaldehyde | at pH 6.5 and 65°C | Yokenella sp. WZY002 | |
0.6 | - |
NADP+ | with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
0.7 | - |
NADP+ | with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
3.3 | - |
crotonaldehyde | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
9.1 | - |
crotonyl alcohol | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
14 | - |
benzyl alcohol | at pH 6.5 and 65°C | Yokenella sp. WZY002 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 113% activity at 1 mM | Yokenella sp. WZY002 | |
additional information | Ca2+, Na+, Mg2+, Mn2+, and K+ have no significant effect on the enzyme activity | Yokenella sp. WZY002 | |
Zn2+ | dependent on | Yokenella sp. WZY002 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36411 | - |
- |
Yokenella sp. WZY002 |
39000 | - |
2 * 39000, SDS-PAGE | Yokenella sp. WZY002 |
75000 | - |
gel filtration | Yokenella sp. WZY002 |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide and also remains active in 40% (v/v) | Yokenella sp. WZY002 |
Ethanol | the enzyme retains more than 49% of the initial activity in 20% (v/v) ethanol and also remains active in 40% (v/v) | Yokenella sp. WZY002 |
isopropanol | the enzyme retains more than 49% of the initial activity in 20% (v/v) isopropanol and also remains active in 40% (v/v) | Yokenella sp. WZY002 |
Methanol | the enzyme retains more than 49% of the initial activity in 20% (v/v) methanol and also remains active in 40% (v/v) | Yokenella sp. WZY002 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yokenella sp. WZY002 | W6CX26 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Yokenella sp. WZY002 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-oct-2-enal + NADPH + H+ | - |
Yokenella sp. WZY002 | trans-2-octen-1-ol + NADP+ | - |
? | |
1-butanol + NADP+ | 56% activity compared to crotyl alcohol | Yokenella sp. WZY002 | butyraldehyde + NADPH + H+ | - |
? | |
1-propanol + NADP+ | 17.4% activity compared to crotyl alcohol | Yokenella sp. WZY002 | propionaldehyde + NADPH + H+ | - |
? | |
2-hydroxyacetophenone + NADPH + H+ | - |
Yokenella sp. WZY002 | (S)-1,2-phenylethanediol + NADP+ | - |
? | |
acetophenone + NADPH + H+ | - |
Yokenella sp. WZY002 | ? | - |
r | |
alpha-ionone + NADPH + H+ | - |
Yokenella sp. WZY002 | ? | - |
r | |
benzaldehyde + NADPH + H+ | highest reductive activity with benzaldehyde | Yokenella sp. WZY002 | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NADP+ | 41.7% activity compared to crotyl alcohol | Yokenella sp. WZY002 | benzaldehyde + NADPH + H+ | - |
r | |
crotonaldehyde + NADPH + H+ | - |
Yokenella sp. WZY002 | crotonyl alcohol + NADP+ | - |
r | |
crotonyl alcohol + NADP+ | highest oxidative activity with crotyl alcohol | Yokenella sp. WZY002 | crotonaldehyde + NADPH + H+ | - |
r | |
additional information | no activity with NADH or NAD+ | Yokenella sp. WZY002 | ? | - |
? | |
trans-4-phenyl-3-buten-2-one + NADPH + H+ | - |
Yokenella sp. WZY002 | ? | - |
r | |
vanillin + NADPH + H+ | - |
Yokenella sp. WZY002 | vanillic alcohol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39000, SDS-PAGE | Yokenella sp. WZY002 |
homodimer | 2 * 36411, calculated from amino acid sequence | Yokenella sp. WZY002 |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Yokenella sp. WZY002 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
for crotonyl alcohol oxidation | Yokenella sp. WZY002 |
65 | - |
for crotonaldehyde reduction | Yokenella sp. WZY002 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C and 1.5 h at 60°C. The activity loss at 65°C is relatively rapid, and the enzyme retains 30.8% of the initial activity after 30 min of heat treatment | Yokenella sp. WZY002 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93.3 | - |
benzyl alcohol | at pH 6.5 and 65°C | Yokenella sp. WZY002 | |
101 | - |
crotonyl alcohol | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
194 | - |
NADP+ | with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
360 | - |
benzaldehyde | at pH 6.5 and 65°C | Yokenella sp. WZY002 | |
405 | - |
crotonaldehyde | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
433 | - |
NADP+ | with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
for crotonaldehyde reduction | Yokenella sp. WZY002 |
8 | - |
for crotonyl alcohol oxidation | Yokenella sp. WZY002 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Yokenella sp. WZY002 | |
NADPH | - |
Yokenella sp. WZY002 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
benzyl alcohol | at pH 6.5 and 65°C | Yokenella sp. WZY002 | |
11 | - |
crotonyl alcohol | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
122 | - |
crotonaldehyde | at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
278 | - |
NADP+ | with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
720 | - |
NADP+ | with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C | Yokenella sp. WZY002 | |
899 | - |
benzaldehyde | at pH 6.5 and 65°C | Yokenella sp. WZY002 |