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Literature summary for 1.1.1.91 extracted from
- Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767 (2012), BMC Microbiol., 12, 126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Phanerodontia chrysosporium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dimethoxybenzaldehyde | - |
Phanerodontia chrysosporium |  |
| 3,4-dimethoxybenzyl alcohol | - |
Phanerodontia chrysosporium | |
| 3,5-dimethoxybenzaldehyde | - |
Phanerodontia chrysosporium | |
| 3-hydroxy-4-methoxy-benzaldehyde | - |
Phanerodontia chrysosporium | |
| 4-hydroxy-3-methoxybenzaldehyde | - |
Phanerodontia chrysosporium | |
| 5-(hydroxymethyl)-2-furaldehyde | - |
Phanerodontia chrysosporium | |
| benzaldehyde | - |
Phanerodontia chrysosporium | |
| trans-cinnamaldehyde | - |
Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.022 | - |
3,5-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.038 | - |
NADP+ | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.039 | - |
NADPH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.09 | - |
4-methoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.22 | - |
NADH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.27 | - |
5-(hydroxymethyl)-2-furaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.37 | - |
3,4-dimethoxybenzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 0.53 | - |
phenylacetaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 1.4 | - |
4-Hydroxy-3-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 1.4 | - |
3-hydroxy-4-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 1.7 | - |
benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.4 | - |
trans-cinnamaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 25 | - |
4-hydroxy-3-methoxy-benzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-fluorobenzaldehyde + NADPH | relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 2-fluorobenzyl alcohol + NADP+ | - |
r | |
| 2-methoxybenzaldehyde + NADPH | relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 2-methoxybenzyl alcohol + NADP+ | - |
r | |
| 2-nitrobenzaldehyde + NADPH | relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 2-nitrobenzyl alcohol + NADP+ | - |
r | |
| 3,4-dimethoxybenzaldehyde + NADH | - |
Phanerodontia chrysosporium | 3,4-dimethoxybenzyl alcohol + NAD+ | - |
r | |
| 3,4-dimethoxybenzaldehyde + NADPH | relative activity: 100% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3,4-dimethoxybenzyl alcohol + NADP+ | - |
r | |
| 3,5-dimethoxybenzaldehyde + NADPH | relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3,5-dimethoxybenzyl alcohol + NADP+ | - |
r | |
| 3-chlorobenzaldehyde + NADPH | relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3-chlorobenzyl alcohol + NADP+ | - |
r | |
| 3-fluorobenzaldehyde + NADPH | relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3-fluorobenzyl alcohol + NADP+ | - |
r | |
| 3-hydroxy-4-methoxy-benzaldehyde + NADPH | relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3-hydroxy-4-methoxy-benzyl alcohol + NADP+ | - |
r | |
| 3-methoxybenzaldehyde + NADPH | relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3-methoxybenzyl alcohol + NADP+ | - |
r | |
| 3-nitrobenzaldehyde + NADPH | relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 3-nitrobenzyl alcohol + NADP+ | - |
r | |
| 4-chlorobenzaldehyde + NADPH | relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-chlorobenzyl alcohol + NADP+ | - |
r | |
| 4-fluorobenzaldehyde + NADPH | relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-fluorobenzyl alcohol + NADP+ | - |
r | |
| 4-hydroxy-3-methoxy-benzaldehyde + NADPH | relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-hydroxy-3-methoxy-benzyl alcohol + NADP+ | - |
r | |
| 4-hydroxybenzaldehyde + NADPH | relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-hydroxybenzyl alcohol + NADP+ | - |
r | |
| 4-methoxybenzaldehyde + NADPH | relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-methoxybenzyl alcohol + NADP+ | - |
r | |
| 4-nitrobenzaldehyde + NADPH | relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | 4-nitrobenzyl alcohol + NADP+ | - |
r | |
| 5-(hydroxymethyl)-2-furaldehyde + NADPH + H+ | relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | (furan-2,5-diyl)dimethanol + NADP+ | - |
r | |
| benzaldehyde + NADPH | relative activity: 42% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | benzyl alcohol + NADP+ | - |
r | |
| decanal + NADPH | relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | decanol + NADP+ | - |
r | |
| heptaldehyde + NADPH | relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | ? | - |
r | |
| hexanal + NADPH | relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | hexanol + NADP+ | - |
r | |
| nonanal + NADPH | relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | nonanol + NADP+ | - |
r | |
| octanal + NADPH | relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | octanol + NADP+ | - |
r | |
| pentanal + NADPH | relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | pentanol + NADP+ | - |
r | |
| phenylacetaldehyde + NADPH | relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | phenylethanol + NADP+ | - |
r | |
| trans-cinnamaldehyde + NADPH | relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde) | Phanerodontia chrysosporium | ? | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aad1p | - |
Phanerodontia chrysosporium |
| aryl-alcohol dehydrogenase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
NADH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.55 | - |
3,4-dimethoxybenzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.56 | - |
NADP+ | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.93 | - |
5-(hydroxymethyl)-2-furaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.33 | - |
4-Hydroxy-3-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.83 | - |
3-hydroxy-4-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 4.33 | - |
4-hydroxy-3-methoxy-benzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 7.16 | - |
benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 8.16 | - |
4-methoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 8.833 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 9.83 | - |
3,5-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 11.16 | - |
phenylacetaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 11.16 | - |
trans-cinnamaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 11.33 | - |
NADPH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
reduction activity | Phanerodontia chrysosporium |
| 10.4 | - |
oxidation activity | Phanerodontia chrysosporium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
3,5-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.3 | - |
3-hydroxy-4-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.4 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.5 | - |
trans-cinnamaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 5.1 | - |
4-hydroxy-3-methoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 81 | - |
benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 136 | - |
5-(hydroxymethyl)-2-furaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 165 | - |
3,4-dimethoxybenzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.166 | - |
4-hydroxy-3-methoxy-benzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.33 | - |
4-Hydroxy-3-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 2.66 | - |
3-hydroxy-4-methoxy-benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 3.33 | - |
trans-cinnamaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 5 | - |
benzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 6.8 | - |
3,4-dimethoxybenzyl alcohol | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 10 | - |
NADH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 10 | - |
5-(hydroxymethyl)-2-furaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 11.16 | - |
phenylacetaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 68 | - |
NADP+ | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 90 | - |
4-methoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 283 | - |
NADPH | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 450 | - |
3,5-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
| 733 | - |
3,4-dimethoxybenzaldehyde | pH 6.1, 30°C | Phanerodontia chrysosporium | |
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