Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.9 extracted from

  • Cunha, J.T.; Soares, P.O.; Romani, A.; Thevelein, J.M.; Domingues, L.
    Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways (2019), Biotechnol. Biofuels, 12, 20 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Saccharomyces cerevisiae strain CA11 (isolated from Brazilian Cachaca fermentation processes) and in strain PE-2DELTAGRE3 (isolated from Brazilian first generation bioethanol plants), coepxression with xylose reductase (XR) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information Saccharomyces cerevisiae, the preferred microorganism for large-scale ethanol production, does not naturally consume xylose. Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways are introduced into two robust industrial Saccharomyces cerevisiae strains, PE-2DELTAGRE3 and CA11, evaluated in synthetic media and corn cob hemicellulosic hydrolysate (non-detoxified corn cob hydrolysate) and the results are correlated with the differential enzyme activities found in the xylose-pathway engineered strains, evaluation of xylose fermentation capacity in oxygen-deprived conditions at 30°C and 40°C. Regarding the CA11-derived strains, the XR activity is higher in the strain with both pathways than in the one solely expressing XR/XDH, while the XDH activity at 30°C is higher in the CA11-XR/XDH strain. The sole expression of XI increases the fermentative capacity of both strains in synthetic media at 30°C and 40°C: decreasing xylitol accumulation and improving xylose consumption and ethanol production. Similar results are observed in fermentations of detoxified hydrolysate. However, in the presence of lignocellulosic-derived inhibitors, a positive synergistic effect results from the expression of both XI and XR/XDH, possibly caused by a cofactor equilibrium between the XDH and furan detoxifying enzymes, increasing the ethanol yield by more than 38%. Advantage of using the XI from Clostridium phytofermentans to attain high ethanol productivities and yields from xylose, and the simultaneous utilization of XR/XDH and XI pathways compared to the single expression of XR/XDH or XI improves ethanol production from non-detoxified hemicellulosic hydrolysates Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Saccharomyces cerevisiae
-
D-xylulose + NADH + H+
-
r
xylitol + NAD+ Saccharomyces cerevisiae ATCC 204508
-
D-xylulose + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q07993
-
-
Saccharomyces cerevisiae ATCC 204508 Q07993
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Saccharomyces cerevisiae D-xylulose + NADH + H+
-
r
xylitol + NAD+
-
Saccharomyces cerevisiae ATCC 204508 D-xylulose + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
XDH
-
Saccharomyces cerevisiae
XYL2
-
Saccharomyces cerevisiae
xylitol dehydrogenase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae
NADH
-
Saccharomyces cerevisiae