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Literature summary for 1.1.1.9 extracted from

  • Zhang, G.C.; Turner, T.L.; Jin, Y.S.
    Enhanced xylose fermentation by engineered yeast expressing NADH oxidase through high cell density inoculums (2017), J. Ind. Microbiol. Biotechnol., 44, 387-395 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information engineered Sacchyromyces cerevisiae expressing NADPH-linked xylose reductase (XR) and NAD+-linked xylitol dehydrogenase (XDH) produces substantial amounts of the reduced byproducts under anaerobic conditions due to the cofactor difference of XR and XDH. While the additional expression of a water-forming NADH oxidase (NoxE) from Lactococcus lactis in engineered Saccharomyces cerevisiae with the XR/XDH pathway leads to reduced glycerol and xylitol production and increased ethanol yields from xylose, volumetric ethanol productivities by the engineered yeast decrease because of growth defects from the overexpression of noxE. High cell density inoculum for xylose fermentation by strain SR8 expressing noxE, resulting in strain SR8N, shows a higher ethanol yield and lower byproduct yields, and also exhibits a high ethanol productivity during xylose fermentation. Growth defects from noxE overexpression can be overcome in the case of fermenting lignocellulose-derived sugars such as glucose and xylosen Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Scheffersomyces stipitis
-
D-xylulose + NADH + H+
-
?
xylitol + NAD+ Scheffersomyces stipitis ATCC 58785
-
D-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis P22144 formerly Pichia stipitis
-
Scheffersomyces stipitis ATCC 58785 P22144 formerly Pichia stipitis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Scheffersomyces stipitis D-xylulose + NADH + H+
-
?
xylitol + NAD+
-
Scheffersomyces stipitis ATCC 58785 D-xylulose + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
NAD+-linked xylitol dehydrogenase
-
Scheffersomyces stipitis
XDH
-
Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Scheffersomyces stipitis