Cloned (Comment) | Organism |
---|---|
gene xdh, cloning from strain CGMCC 1.49, DNA and amino acid sequence determination and analysis, functional recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21 | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Gluconobacter oxydans | - |
D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | Gluconobacter oxydans CGMCC 1.49 | - |
D-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | A0A141BGH5 | - |
- |
Gluconobacter oxydans CGMCC 1.49 | A0A141BGH5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Gluconobacter oxydans |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
purified native enzyme, substrate xylitol, pH 7.0, 30°C | Gluconobacter oxydans |
14.2 | - |
purified recombinant His6-tagged enzyme, substrate D-mannitol, pH 11.0, 30°C | Gluconobacter oxydans |
14.2 | - |
purified recombinant His6-tagged enzyme, substrate xylitol, pH 7.0, 30°C | Gluconobacter oxydans |
87.8 | - |
purified recombinant His6-tagged enzyme, substrate xylitol, pH 11.0, 30°C | Gluconobacter oxydans |
135.2 | - |
purified recombinant His6-tagged enzyme, substrate D-sorbitol, pH 11.0, 30°C | Gluconobacter oxydans |
151.4 | - |
purified recombinant His6-tagged enzyme, substrate D-fructose, pH 11.0, 30°C | Gluconobacter oxydans |
326.3 | - |
purified recombinant His6-tagged enzyme, substrate D-xylulose, pH 11.0, 30°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | low activity compared to xylitol | Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
D-mannitol + NAD+ | low activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | D-fructose + NADH + H+ | - |
r | |
D-sorbitol + NAD+ | 51.3% increased activity compared to xylitol | Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
? | |
D-sorbitol + NAD+ | 51.3% increased activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | L-sorbose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Gluconobacter oxydans CGMCC 1.49 | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Gluconobacter oxydans CGMCC 1.49 | D-xylulose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, yes recombinant His6-tagged enzyme, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
XDH | - |
Gluconobacter oxydans |
xylitol dehydrogenase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
oxidation of xylitol | Gluconobacter oxydans |
35 | - |
reduction of D-xylulose | Gluconobacter oxydans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | over 80% of maximal activity within this range for both reaction directions | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of D-xylulose | Gluconobacter oxydans |
11 | - |
oxidation of xylitol | Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | over 60% of maximal activity within this range for oxidation of xylitol, profile overview | Gluconobacter oxydans |
10 | 12 | over 60% of maximal activity within this range for reduction of D-xylulose, profile overview | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gluconobacter oxydans |
General Information | Comment | Organism |
---|---|---|
evolution | XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/ reductase family | Gluconobacter oxydans |