Literature summary for 1.1.1.9 extracted from

Nidetzky, B.; Helmer, H.; Klimacek, M.; Lunzer, R.; Mayer, G.
Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli (2003), Chem. Biol. Interact., 143-144, 533-542.

Search for more literature for 1.1.1.9

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no significant content of Mg2+	Candida sp. HA 167
Zn2+	0.9 molecules per subunit	Candida sp. HA 167

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	4 * 38000, SDS-PAGE	Candida sp. HA 167
160000	-	gel filtration	Candida sp. HA 167

Organism

Organism	UniProt	Comment	Textmining
Candida sp. HA 167	-	expressed in Escherichia coli	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Candida sp. HA 167

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose + NADH + H+	-	Candida sp. HA 167	D-sorbitol + NAD+	-	?
D-sorbitol + NAD+	-	Candida sp. HA 167	D-fructose + NADH + H+	-	r
D-xylitol + NAD+	-	Candida sp. HA 167	D-xylulose + NADH + H+	-	r
D-xylulose + NADH + H+	specific for transferring the 4-pro-R hydrogen of NADH	Candida sp. HA 167	D-xylitol + NAD+	-	r
additional information	specific for transferring the 4-pro-R hydrogen of NADH	Candida sp. HA 167	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 38000, SDS-PAGE	Candida sp. HA 167

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
114	-	D-sorbitol	25°C, pH 7.5, Tris-HCl	Candida sp. HA 167
143	-	xylitol	25°C, pH 7.5, Tris-HCl	Candida sp. HA 167
170	-	xylitol	25°C, pH 7.5, potassium phosphate	Candida sp. HA 167
920	-	D-fructose	25°C, pH 7.5, Tris-HCl	Candida sp. HA 167
1500	-	D-xylulose	25°C, pH 7.5, Tris-HCl	Candida sp. HA 167
1800	-	D-xylulose	25°C, pH 7.5, potassium phosphate	Candida sp. HA 167

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Candida sp. HA 167
NADH	-	Candida sp. HA 167

pI Value

Organism	Comment	pI Value Maximum	pI Value
Candida sp. HA 167	isoelectric focusing	-	6

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Release 2023.1 (January 2023)