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Literature summary for 1.1.1.86 extracted from

  • Chen, C.Y.; Chang, Y.C.; Lin, B.L.; Lin, K.F.; Huang, C.H.; Hsieh, D.L.; Ko, T.P.; Tsai, M.D.
    Use of cryo-EM To uncover structural bases of pH effect and cofactor bispecificity of ketol-acid reductoisomerase (2019), J. Am. Chem. Soc., 141, 6136-6140 .
    View publication on PubMed
Search for more literature for 1.1.1.86

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 NADPH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.15
-
 NADH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
2.69
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
4.43
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
440000
-
-
 Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-acetolactate + NADH + H+
-
 Saccharolobus solfataricus (R)-2,3-dihydroxyisovalerate + NAD+
-
 ?
(S)-2-acetolactate + NADPH + H+
-
 Saccharolobus solfataricus (R)-2,3-dihydroxyisovalerate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dodecamer the dodecameric architecture is assembled by six dimeric units which are positioned on the tetrahedron edges to form a tetrahedron with T symmetry Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
KARI
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 80 activity increases from 40 to 80°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 85 stable, based on CD analysis Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 NADPH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.01
-
 NADH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.011
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.013
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 stable, based on CD analysis Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme is bispecific to NADH and NADPH Saccharolobus solfataricus
NADPH the enzyme is bispecific to NADH and NADPH Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0029
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH Saccharolobus solfataricus
0.00383
-
 (S)-2-acetolactate pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.0631
-
 NADPH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus
0.0714
-
 NADH pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH Saccharolobus solfataricus