Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
((4-methoxyphenyl)carbamoyl) (5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl)amide | - |
Mycobacterium tuberculosis | |
N-(4-chlorophenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | low toxicity against HEK 293T cell lines | Mycobacterium tuberculosis | |
N-(4-methoxyphenyl)-N'-[5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | low toxicity against HEK 293T cell lines | Mycobacterium tuberculosis | |
N-(4-methoxyphenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | low toxicity against HEK 293T cell lines | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | two magnesium ions are located deep in the active site pocket and are separated by 4.7 A. The side chains of D188 and E192, and four water molecules form ligands with one of the Mg2+ ions, Mg2+(I), and the second Mg2+ ion, Mg2+ (II), is liganded also by the side-chain of D188, in addition to E224 and E228 and three water molecules | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKJ7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis | 3-hydroxy-3-methyl-2-oxobutyrate + NADP+ | - |
? | |
(2S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | 3-hydroxy-3-methyl-2-oxobutyrate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00202 | - |
N-(4-methoxyphenyl)-N'-[5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00306 | - |
((4-methoxyphenyl)carbamoyl) (5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl)amide | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00472 | - |
N-(4-chlorophenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00548 | - |
N-(4-methoxyphenyl)-N'-[5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00835 | - |
N-(4-chlorophenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00927 | - |
N-(4-methoxyphenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea | pH 8.0, 25°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
drug target | anti-tuberculosis drug target | Mycobacterium tuberculosis |
metabolism | the enzyme is involved in the branched chain amino acid biosynthesis pathway | Mycobacterium tuberculosis |