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Literature summary for 1.1.1.86 extracted from

  • Kim, G.; Shin, D.; Lee, S.; Yun, J.; Lee, S.
    Crystal structure of ILvC, a ketol-acid reductoisomerase, from Streptococcus pneumoniae (2019), Crystals, 9, 551 .
No PubMed abstract available
Search for more literature for 1.1.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
micro-batch and hanging-drop vapor-diffusion methods. The crystal structure is determined at 1.69 A resolution. The crystal structure of SpIlvC contains an asymmetric dimer in which one subunit is in apo-form and the other in NADP(H) and Mg2+-bound form Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
D191G crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
D191K mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
D83G crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
E195A mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
E195K mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
E195S crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
R49A mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
R49E crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
R49G mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
S53A mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
S53G mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
S53K mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae
S53T mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADPH wild-type enzyme, pH 8.0, 30°C Streptococcus pneumoniae
0.199
-
 NADPH mutant enzyme S53G, pH 8.0, 30°C Streptococcus pneumoniae
0.33
-
 NADPH mutant enzyme D83G, pH 8.0, 30°C Streptococcus pneumoniae
0.345
-
 NADPH mutant enzyme S53T, pH 8.0, 30°C Streptococcus pneumoniae
0.355
-
 NADPH mutant enzyme D191G, pH 8.0, 30°C Streptococcus pneumoniae
0.395
-
 NADPH mutant enzyme R49G, pH 8.0, 30°C Streptococcus pneumoniae
0.47 1 NADPH mutant enzyme S53K, pH 8.0, 30°C Streptococcus pneumoniae
0.472
-
 NADPH mutant enzyme R49E, pH 8.0, 30°C Streptococcus pneumoniae
0.588
-
 NADPH mutant enzyme R49A, pH 8.0, 30°C Streptococcus pneumoniae
0.677
-
 NADPH mutant enzyme S53A, pH 8.0, 30°C Streptococcus pneumoniae
0.732
-
 NADPH mutant enzyme E195A, pH 8.0, 30°C Streptococcus pneumoniae
0.895
-
 NADPH mutant enzyme E195K, pH 8.0, 30°C Streptococcus pneumoniae
1.036
-
 NADPH mutant enzyme D191K, pH 8.0, 30°C Streptococcus pneumoniae
1.467
-
 NADPH mutant enzyme E195S, pH 8.0, 30°C Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q04M32
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-acetolactate + NADPH + H+
-
 Streptococcus pneumoniae (R)-2,3-dihydroxyisovalerate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer the enzyme retains dimeric arrangement in solution Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
ilvC
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 NADPH wild-type enzyme, pH 8.0, 30°C Streptococcus pneumoniae
0.11
-
 NADPH mutant enzyme S53G, pH 8.0, 30°C Streptococcus pneumoniae
0.12
-
 NADPH mutant enzyme D191K, pH 8.0, 30°C Streptococcus pneumoniae
0.12
-
 NADPH mutant enzyme E195A, pH 8.0, 30°C Streptococcus pneumoniae
0.12
-
 NADPH mutant enzyme S53T, pH 8.0, 30°C Streptococcus pneumoniae
0.13
-
 NADPH mutant enzyme D83G, pH 8.0, 30°C Streptococcus pneumoniae
0.14
-
 NADPH mutant enzyme S53K, pH 8.0, 30°C Streptococcus pneumoniae
0.15
-
 NADPH mutant enzyme D191G, pH 8.0, 30°C Streptococcus pneumoniae
0.16
-
 NADPH mutant enzyme R49G, pH 8.0, 30°C Streptococcus pneumoniae
0.17
-
 NADPH mutant enzyme S53A, pH 8.0, 30°C Streptococcus pneumoniae
0.18
-
 NADPH mutant enzyme E195K, pH 8.0, 30°C Streptococcus pneumoniae
0.18
-
 NADPH mutant enzyme R49A, pH 8.0, 30°C Streptococcus pneumoniae
0.18
-
 NADPH mutant enzyme R49E, pH 8.0, 30°C Streptococcus pneumoniae
0.25
-
 NADPH mutant enzyme E195S, pH 8.0, 30°C Streptococcus pneumoniae

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the second step in the BCAA biosynthetic pathway Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 NADPH mutant enzyme E195A, pH 8.0, 30°C Streptococcus pneumoniae
0.17
-
 NADPH mutant enzyme E195S, pH 8.0, 30°C Streptococcus pneumoniae
0.2
-
 NADPH mutant enzyme E195K, pH 8.0, 30°C Streptococcus pneumoniae
0.21
-
 NADPH mutant enzyme D191K, pH 8.0, 30°C Streptococcus pneumoniae
0.25
-
 NADPH mutant enzyme S53A, pH 8.0, 30°C Streptococcus pneumoniae
0.3
-
 NADPH mutant enzyme R49A, pH 8.0, 30°C Streptococcus pneumoniae
0.3
-
 NADPH mutant enzyme S53K, pH 8.0, 30°C Streptococcus pneumoniae
0.34
-
 NADPH mutant enzyme S53T, pH 8.0, 30°C Streptococcus pneumoniae
0.38
-
 NADPH mutant enzyme R49E, pH 8.0, 30°C Streptococcus pneumoniae
0.39
-
 NADPH mutant enzyme D83G, pH 8.0, 30°C Streptococcus pneumoniae
0.42
-
 NADPH mutant enzyme D191G, pH 8.0, 30°C Streptococcus pneumoniae
0.43
-
 NADPH mutant enzyme R49G, pH 8.0, 30°C Streptococcus pneumoniae
0.55
-
 NADPH mutant enzyme S53G, pH 8.0, 30°C Streptococcus pneumoniae
1.4
-
 NADPH wild-type enzyme, pH 8.0, 30°C Streptococcus pneumoniae