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Literature summary for 1.1.1.86 extracted from

  • Wun, S.J.; Johnson, L.A.; You, L.; McGeary, R.P.; Brueck, T.; Schenk, G.; Guddat, L.W.
    Inhibition studies of ketol-acid reductoisomerases from pathogenic microorganisms (2020), Arch. Biochem. Biophys., 692, 108516 .
    View publication on PubMed
Search for more literature for 1.1.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Campylobacter jejuni
expression in Escherichia coli BL21 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
2-dimethylphosphoryl-2-hydroxy-acetic acid i.e. Hoe 704, reversible inhibitor Campylobacter jejuni
2-dimethylphosphoryl-2-hydroxy-acetic acid i.e. Hoe 704, reversible inhibitor Mycobacterium tuberculosis
cyclopropane-1,1-dicarboxylate reversible inhibitor Campylobacter jejuni
cyclopropane-1,1-dicarboxylate reversible inhibitor Mycobacterium tuberculosis
N-Hydroxy-N-isopropyloxamate irreversible Campylobacter jejuni
N-Hydroxy-N-isopropyloxamate
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
0.58
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
2.4
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni
6
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Mycobacterium tuberculosis P9WKJ7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WKJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Campylobacter jejuni
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
 substrate: hydroxypyruvate, pH 8.0, 25°C Mycobacterium tuberculosis
0.5
-
 substrate: hydroxypyruvate, pH 8.0, 25°C Campylobacter jejuni
1.4
-
 substrate: 2-acetolactate, pH 8.0, 25°C Campylobacter jejuni
6
-
 substrate: (2S)-2-acetolactate, pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
(2S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
2-acetolactate + NADPH
-
 Campylobacter jejuni 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
hydroxypyruvate + NADPH + H+
-
 Campylobacter jejuni glycerate + NADP+
-
 ?
hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis glycerate + NADP+
-
 ?
hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 glycerate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Mycobacterium tuberculosis
dodecamer
-
 Campylobacter jejuni

Synonyms

Synonyms Comment Organism
KARI
-
 Campylobacter jejuni
KARI
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
0.3
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
0.7
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni
3.7
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000026
-
 N-Hydroxy-N-isopropyloxamate pH 8.0, 25°C Campylobacter jejuni
0.000026
-
 N-Hydroxy-N-isopropyloxamate pH 8.0, 25°C Mycobacterium tuberculosis
0.0003
-
 2-dimethylphosphoryl-2-hydroxy-acetic acid pH 8.0, 25°C Campylobacter jejuni
0.0003
-
 2-dimethylphosphoryl-2-hydroxy-acetic acid pH 8.0, 25°C Mycobacterium tuberculosis
0.00303
-
 cyclopropane-1,1-dicarboxylate pH 8.0, 25°C Campylobacter jejuni
0.00303
-
 cyclopropane-1,1-dicarboxylate pH 8.0, 25°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
drug target target for the discovery of biocides Campylobacter jejuni
drug target target for the discovery of biocides Mycobacterium tuberculosis
metabolism the enzyme is involved in the branched-chain amino acid biosynthesis Campylobacter jejuni
metabolism the enzyme is involved in the branched-chain amino acid biosynthesis Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.55
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
0.63
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis
0.77
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
27
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni