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Literature summary for 1.1.1.86 extracted from
- Cyclopropane-1,1-dicarboxylate is a slow-, tight-binding inhibitor of rice ketol-acid reductoisomerase (2005), Plant Sci., 168, 1035-1040.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-aminocarbonylcyclopropanecarboxylate | - |
Oryza sativa |  |
| 1-cyanocyclopropanecarboxylate | - |
Oryza sativa | |
| 1-hydroxycyclopropanecarboxylate | - |
Oryza sativa | |
| cyclopropane-1,1-dicarboxylate | a competitive slow-, tight-binding inhibitor | Oryza sativa | |
| dimethylmalonate | slow-binding inhibitor | Oryza sativa | |
| additional information | no inhibition by 1-aminocyclopropanecarboxylate, 1-methylcyclopropanecarboxylate, 2-methylcyclopropanecarboxylate, cyclopropane-1,2-dicarboxylate, and cyclopropanecarboxylate dimethyl ester | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0072 | - |
NADPH | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
| 0.042 | - |
2-acetolactate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
| 0.893 | - |
Hydroxypyruvate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, KM: 0.00324 mM | Oryza sativa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q65XK0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
purified recombinant enzyme, substrate 2-acetolactate | Oryza sativa |
| 1.97 | - |
purified recombinant enzyme, substrate hydroxypyruvate | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | - |
Oryza sativa | 2,3-dihydroxyisovalerate + NADP+ | - |
? | |
| hydroxypyruvate + NADPH | - |
Oryza sativa | glycerate + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KARI | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Oryza sativa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition mechanism and kinetics | Oryza sativa | |
| 0.0000903 | - |
cyclopropane-1,1-dicarboxylate | pH 8.0, 30°C, recombinant enzyme, versus hydroxypyruvate | Oryza sativa | |
| 0.000716 | - |
dimethylmalonate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
| 0.0056 | - |
1-hydroxycyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
| 0.0203 | - |
1-aminocarbonylcyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
| 0.0585 | - |
1-cyanocyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
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