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Literature summary for 1.1.1.86 extracted from

  • Biou, V.; Dumas, R.; Cohen-Addad, C.; Douce, R.; Job, D.; Pebay-Peyroula, E.
    The crystal structure of plant acetohydroxy acid isomeroreductase complexed with NADPH, two magnesium ions and a herbicidal transition state analog determined at 1.65 A resolution (1997), EMBO J., 16, 3405-3415.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation, crystal structure of the enzyme complexed with NADPH, two magnesium ions and N-hydroxy-N-isopropyloxamate, a herbicidal transition state analog determined at 1.65 A resolution, recombinant enzyme overexpressed in Escherichia coli Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
N-Hydroxy-N-isopropyloxamate competitive Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Spinacia oleracea
Mg2+ two ions bind to enzyme Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetolactate + NADPH Spinacia oleracea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 overexpressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
NADPH + 2-acetolactate
-
 Spinacia oleracea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Spinacia oleracea