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Literature summary for 1.1.1.86 extracted from
- Ketol-acid reductoisomerase from barley (Hordeum vulgare). Purification, properties, and specific inhibition (1993), Plant Physiol., 103, 903-910.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-dimethylphosphinoyl-2-hydroxyacetic acid | competitive, reversible | Hordeum vulgare |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0043 | - |
NADPH | pH 7.5, 25°C | Hordeum vulgare | |
| 0.011 | - |
2-acetolactate | pH 7.5, 25°C | Hordeum vulgare | |
| 0.038 | - |
2-aceto-2-hydroxybutyrate | pH 7.5, 25°C | Hordeum vulgare | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
2 * 59000, SDS-PAGE | Hordeum vulgare |
| 115000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | Hordeum vulgare | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.04 | - |
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aceto-2-hydroxybutyrate + NADPH + H+ | - |
Hordeum vulgare | 2,3-dihydroxy-3-methylvalerate + NADP+ | - |
r | |
| 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Hordeum vulgare | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| NADPH + 2-acetolactate | - |
Hordeum vulgare | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 59000, SDS-PAGE | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
2-acetolactate as substrate | Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Hordeum vulgare | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00019 | - |
2-dimethylphosphinoyl-2-hydroxyacetic acid | pH 7.5, 25°C, with 2-aceto-2-hydroxybutyrate as substrate | Hordeum vulgare | |
| 0.00046 | - |
2-dimethylphosphinoyl-2-hydroxyacetic acid | pH 7.5, 25°C, with 2-acetolactate as substrate | Hordeum vulgare | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | chromatofocusing | 4.7 | 4.6 |
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