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Literature summary for 1.1.1.86 extracted from
- The pH dependence of the kinetic parameters of ketol acid reductoisomerase indicates a proton shuttle mechanism for alkyl migration (1992), Arch. Biochem. Biophys., 294, 446-453.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Methyllactate | competitive | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Mg2+ | Km: 0.4 mM, pH 8.5 | Escherichia coli | |
| Mg2+ | Km: 0.35 mM, pH 10, reaction with acetolactate | Escherichia coli | |
| Mg2+ | Km: 0.45 mM, pH 6.7, reaction with acetolactate | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | Escherichia coli | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 2-acetolactate + NADPH | Escherichia coli | second common reaction in the biosynthesis of the branched chain amino acids | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 2-acetolactate + NADPH | second common reaction in the biosynthesis of the branched chain amino acids | Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| acetolactate + NADPH + H+ | - |
Escherichia coli | 2,3-dihydroxy-2-methylbutanoate + NADP+ | - |
r | |
| NADPH + 2-acetolactate | - |
Escherichia coli | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
rapid loss of activity above pH 10 with 2-acetolactate as substrate | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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