Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Methyllactate | competitive | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | Km: 0.4 mM, pH 8.5 | Escherichia coli | |
Mg2+ | Km: 0.35 mM, pH 10, reaction with acetolactate | Escherichia coli | |
Mg2+ | Km: 0.45 mM, pH 6.7, reaction with acetolactate | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Escherichia coli | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | Escherichia coli | second common reaction in the biosynthesis of the branched chain amino acids | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | second common reaction in the biosynthesis of the branched chain amino acids | Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
acetolactate + NADPH + H+ | - |
Escherichia coli | 2,3-dihydroxy-2-methylbutanoate + NADP+ | - |
r | |
NADPH + 2-acetolactate | - |
Escherichia coli | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
rapid loss of activity above pH 10 with 2-acetolactate as substrate | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |