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Literature summary for 1.1.1.86 extracted from
- A kinetic study of alpha-acetohydroxy acid isomeroreductase from Salmonella typhimurium (1973), Arch. Biochem. Biophys., 158, 132-138.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxyisovalerate | noncompetitive | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| NADP+ | linear non-competitive with respect to 2-acetolactate, linear competitive inhibitor of NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Salmonella enterica subsp. enterica serovar Typhimurium | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| NADPH + 2-acetolactate | ordered reaction with NADPH and the acetohydroxy acid adding in that order, and dihydroxy acid released before NADP+. 4 mol of NADPH bind per mol of enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | 4 mol of NADPH bind per mol of enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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Release 2023.1 (January 2023)
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