Protein Variants | Comment | Organism |
---|---|---|
V126A | the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126E | the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126F | the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126G | the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126I | the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126L | the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126M | the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126S | the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
V126T | the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126G | Thermus thermophilus | |
0.0026 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126A | Thermus thermophilus | |
0.0027 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126T | Thermus thermophilus | |
0.0032 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126S | Thermus thermophilus | |
0.0044 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, wild-type | Thermus thermophilus | |
0.005 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126M | Thermus thermophilus | |
0.0061 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126E | Thermus thermophilus | |
0.0084 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126I | Thermus thermophilus | |
0.0091 | - |
NAD+ | pH 8.0, 40°C, mutant V126S | Thermus thermophilus | |
0.0092 | - |
NAD+ | pH 8.0, 40°C, mutant V126A | Thermus thermophilus | |
0.0121 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126L | Thermus thermophilus | |
0.0133 | - |
DL-3-isopropylmalate | pH 8.0, 60°C, mutant V126F | Thermus thermophilus | |
0.022 | - |
NAD+ | pH 8.0, 40°C, mutant V126T | Thermus thermophilus | |
0.024 | - |
NAD+ | pH 8.0, 40°C, mutant V126G | Thermus thermophilus | |
0.025 | - |
NAD+ | pH 8.0, 40°C, wild-type | Thermus thermophilus | |
0.0515 | - |
NAD+ | pH 8.0, 40°C, mutant V126E | Thermus thermophilus | |
0.059 | - |
NAD+ | pH 8.0, 60°C, mutant V126A | Thermus thermophilus | |
0.062 | - |
NAD+ | pH 8.0, 40°C, mutant V126I | Thermus thermophilus | |
0.0725 | - |
NAD+ | pH 8.0, 60°C, mutant V126G | Thermus thermophilus | |
0.095 | - |
NAD+ | pH 8.0, 60°C, mutant V126S | Thermus thermophilus | |
0.103 | - |
NAD+ | pH 8.0, 60°C, mutant V126T | Thermus thermophilus | |
0.109 | - |
NAD+ | pH 8.0, 60°C, mutant V126E | Thermus thermophilus | |
0.111 | - |
NAD+ | pH 8.0, 40°C, mutant V126L | Thermus thermophilus | |
0.144 | - |
NAD+ | pH 8.0, 40°C, mutant V126F | Thermus thermophilus | |
0.166 | - |
NAD+ | pH 8.0, 60°C, wild-type | Thermus thermophilus | |
0.198 | - |
NAD+ | pH 8.0, 60°C, mutant V126I | Thermus thermophilus | |
0.378 | - |
NAD+ | pH 8.0, 60°C, mutant V126F | Thermus thermophilus | |
0.391 | - |
NAD+ | pH 8.0, 60°C, mutant V126L | Thermus thermophilus | |
1.01 | - |
NAD+ | pH 8.0, 40°C, mutant V126M | Thermus thermophilus | |
1.97 | - |
NAD+ | pH 8.0, 60°C, mutant V126M | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | DL-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-isopropylmalate dehydrogenase | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126 | Thermus thermophilus | |
1.2 | - |
NAD+ | pH 8.0, 40°C, mutant V126A | Thermus thermophilus | |
1.5 | - |
NAD+ | pH 8.0, 40°C, mutant V126S | Thermus thermophilus | |
1.7 | - |
NAD+ | pH 8.0, 40°C, mutant V126G | Thermus thermophilus | |
1.9 | - |
NAD+ | pH 8.0, 40°C, mutant V126T | Thermus thermophilus | |
2.4 | - |
NAD+ | pH 8.0, 40°C, wild-type | Thermus thermophilus | |
3.5 | - |
NAD+ | pH 8.0, 40°C, mutant V126E | Thermus thermophilus | |
6 | - |
NAD+ | pH 8.0, 40°C, mutant V126I | Thermus thermophilus | |
8.5 | - |
NAD+ | pH 8.0, 40°C, mutant V126L | Thermus thermophilus | |
9 | - |
NAD+ | pH 8.0, 60°C, mutant V126A | Thermus thermophilus | |
10 | - |
NAD+ | pH 8.0, 60°C, V126G | Thermus thermophilus | |
11 | - |
NAD+ | pH 8.0, 40°C, mutant V126F | Thermus thermophilus | |
13 | - |
NAD+ | pH 8.0, 60°C, mutant V126S | Thermus thermophilus | |
15 | - |
NAD+ | pH 8.0, 60°C, mutant V126T | Thermus thermophilus | |
18 | - |
NAD+ | pH 8.0, 40°C, mutant V126M | Thermus thermophilus | |
20 | - |
NAD+ | pH 8.0, 60°C, wild-type | Thermus thermophilus | |
21 | - |
NAD+ | pH 8.0, 60°C, mutant V126E | Thermus thermophilus | |
30.5 | - |
NAD+ | pH 8.0, 60°C, mutant V126I | Thermus thermophilus | |
42 | - |
NAD+ | pH 8.0, 60°C, mutant V126F | Thermus thermophilus | |
44.5 | - |
NAD+ | pH 8.0, 60°C, mutant V126L | Thermus thermophilus | |
54 | - |
NAD+ | pH 8.0, 60°C, mutant V126M | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |