KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
isopropylmalate | pH 7.6, 60°C | Thermus thermophilus | |
0.09 | - |
ethylmalate | pH 7.6 | Escherichia coli | |
0.105 | - |
propylmalate | pH 7.6 | Escherichia coli | |
0.105 | - |
isopropylmalate | pH 7.6, 40°C | Escherichia coli | |
0.139 | - |
NAD+ | pH 7.6, 60°C | Thermus thermophilus | |
0.157 | - |
NAD+ | pH 7.6, 40°C | Thermus thermophilus | |
0.214 | - |
tert-butylmalate | pH 7.6 | Escherichia coli | |
0.316 | - |
isopropylmalate | pH 7.6, 40°C | Thermus thermophilus | |
0.321 | - |
NAD+ | pH 7.6, 40°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Thermus thermophilus | |
K+ | 0.3 mM required for optimal activity | Escherichia coli | |
Mg2+ | - |
Thermus thermophilus | |
Mg2+ | divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+ | Escherichia coli | |
Mn2+ | - |
Thermus thermophilus | |
Mn2+ | divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Escherichia coli | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
ethylmalate + NAD+ | - |
Escherichia coli | 2-oxopentanoate + NADH + H+ + CO2 | - |
? | |
isopropylmalate + NAD+ | - |
Escherichia coli | ? | - |
? | |
propylmalate + NAD+ | - |
Escherichia coli | ? | - |
r | |
tert-butylmalate + NAD+ | - |
Escherichia coli | 2-hydroxy-4,4-dimethylpentanoate + NADH + H+ + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Escherichia coli |
80 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 45% of maximal activity, 80°C: about 85% of maximal activity | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
midpoint of irreversible denaturation | Escherichia coli |
64 | - |
- |
Thermus thermophilus |
64 | - |
10 min, 50% inactivation | Escherichia coli |
79 | - |
midpoint of irreversible denaturation | Thermus thermophilus |
83 | - |
10 min, 50% inactivation | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
tert-butylmalate | pH 7.6 | Escherichia coli | |
8 | - |
isopropylmalate | pH 7.6, 70°C | Thermus thermophilus | |
8 | - |
NAD+ | pH 7.6, 70°C, reaction with isopropylmalate | Thermus thermophilus | |
19 | - |
isopropylmalate | pH 7.6, 40°C | Thermus thermophilus | |
19 | - |
NAD+ | pH 7.6, 40°C, reaction with isopropylmalate | Thermus thermophilus | |
69 | - |
isopropylmalate | pH 7.6, 40°C | Escherichia coli | |
69 | - |
NAD+ | pH 7.6, 40°C, reaction with isopropylmalate | Escherichia coli | |
70 | - |
ethylmalate | pH 7.6 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus | |
NAD+ | - |
Escherichia coli |