Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
iodoacetate | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
NEM | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
PCMB | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
(2R,3S)-3-isopropylmalate | pH 9.0 | Acidithiobacillus ferrooxidans | |
0.8 | - |
NAD+ | pH 9.0 | Acidithiobacillus ferrooxidans | |
4 | - |
D-malate | pH 9.0 | Acidithiobacillus ferrooxidans | |
12 | - |
L-malate | pH 9.0 | Acidithiobacillus ferrooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required | Acidithiobacillus ferrooxidans | |
Mg2+ | 1.0 mM, enhances activity 4.7fold | Acidithiobacillus ferrooxidans | |
Mn2+ | 1.0 mM, enhances activity 4.2fold | Acidithiobacillus ferrooxidans | |
NH4+ | monovalent cation required | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Acidithiobacillus ferrooxidans |
58000 | - |
gel filtration | Acidithiobacillus ferrooxidans |
73000 | - |
equilibrium sedimentation | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
138.2 | - |
- |
Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | 12% of the activity with 3-isopropylmalate | Acidithiobacillus ferrooxidans | pyruvate + NADH + CO2 | - |
? | |
D-malate + NAD+ | 19% of the activity with 3-isopropylmalate | Acidithiobacillus ferrooxidans | pyruvate + NADH + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acidithiobacillus ferrooxidans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 40% loss of activity | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acidithiobacillus ferrooxidans |