Protein Variants | Comment | Organism |
---|---|---|
A172D | enhanced thermostability compared to wild-type enzyme | Thermus thermophilus |
A172E | enhanced thermostability compared to wild-type enzyme | Thermus thermophilus |
A172N | enhanced thermostability compared to wild-type enzyme | Thermus thermophilus |
A172Q | enhanced thermostability compared to wild-type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0169 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172D | Thermus thermophilus | |
0.0169 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A172D, at 55°C, pH not specified in the publication | Thermus thermophilus | |
0.0221 | - |
(2R,3S)-3-isopropylmalate | 55°C, wild-type enzyme | Thermus thermophilus | |
0.0221 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at 55°C, pH not specified in the publication | Thermus thermophilus | |
0.0234 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172N | Thermus thermophilus | |
0.0234 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A172N, at 55°C, pH not specified in the publication | Thermus thermophilus | |
0.0257 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172E | Thermus thermophilus | |
0.0257 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A172E, at 55°C, pH not specified in the publication | Thermus thermophilus | |
0.0267 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172Q | Thermus thermophilus | |
0.0267 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A172Q, at 55°C, pH not specified in the publication | Thermus thermophilus | |
0.076 | - |
NAD+ | 55°C, mutant enzyme A172D | Thermus thermophilus | |
0.094 | - |
NAD+ | 55°C, mutant enzyme A172Q | Thermus thermophilus | |
0.1 | - |
NAD+ | 55°C, mutant enzyme A172E | Thermus thermophilus | |
0.105 | - |
NAD+ | 55°C, mutant enzyme A172N | Thermus thermophilus | |
0.116 | - |
NAD+ | 55°C, wild-type enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
NAD+ | 55°C, mutant enzyme A172D | Thermus thermophilus | |
100 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172D | Thermus thermophilus | |
107 | - |
NAD+ | 55°C, wild-type enzyme | Thermus thermophilus | |
107 | - |
(2R,3S)-3-isopropylmalate | 55°C, wild-type enzyme | Thermus thermophilus | |
111 | - |
NAD+ | 55°C, mutant enzyme A172Q | Thermus thermophilus | |
111 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172Q | Thermus thermophilus | |
117 | - |
NAD+ | 55°C, mutant enzyme A172E and A172N | Thermus thermophilus | |
117 | - |
(2R,3S)-3-isopropylmalate | 55°C, mutant enzyme A172E and A172N | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |