Comparison of D-malate and beta, beta-dimethylmalate dehydrogenases from Pseudomonas fluorescens UK-1

Lähdesmäki, M.; Mäntsälä, P.; Biochim. Biophys. Acta 613, 266-274 (1980)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

1,2-Butanedione

-

Pseudomonas fluorescens

additional information

fairly stable against SH-group reactants

Pseudomonas fluorescens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.15

0.2

3,3-dimethyl-D-malate

-

Pseudomonas fluorescens

0.27

-

NAD+

-

Pseudomonas fluorescens

8.9

11

D-malate

-

Pseudomonas fluorescens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

(R)-3,3-dimethylmalate + NAD+

Pseudomonas fluorescens

-

3-methyl-2-oxobutanoate + CO2 + NADH

-

Pseudomonas fluorescens

?

(R)-3,3-dimethylmalate + NAD+

Pseudomonas fluorescens UK-1

-

3-methyl-2-oxobutanoate + CO2 + NADH

-

Pseudomonas fluorescens UK-1

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

(R)-3,3-dimethylmalate + NAD+

-

207978

Pseudomonas fluorescens

3-methyl-2-oxobutanoate + CO2 + NADH

-

207978

Pseudomonas fluorescens

?

(R)-3,3-dimethylmalate + NAD+

-

207978

Pseudomonas fluorescens UK-1

3-methyl-2-oxobutanoate + CO2 + NADH

-

207978

Pseudomonas fluorescens UK-1

?

D-malate + NAD+

-

207978

Pseudomonas fluorescens

pyruvate + CO2 + NADH

-

207978

Pseudomonas fluorescens

?

D-malate + NAD+

-

207978

Pseudomonas fluorescens UK-1

pyruvate + CO2 + NADH

-

207978

Pseudomonas fluorescens UK-1

?

Cofactor

Commentary

Organism

Structure

NAD+

-

Pseudomonas fluorescens

Cofactor

Commentary

Organism

Structure

NAD+

-

Pseudomonas fluorescens

Inhibitors

Commentary

Organism

Structure

1,2-Butanedione

-

Pseudomonas fluorescens

additional information

fairly stable against SH-group reactants

Pseudomonas fluorescens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.15

0.2

3,3-dimethyl-D-malate

-

Pseudomonas fluorescens

0.27

-

NAD+

-

Pseudomonas fluorescens

8.9

11

D-malate

-

Pseudomonas fluorescens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

(R)-3,3-dimethylmalate + NAD+

Pseudomonas fluorescens

-

3-methyl-2-oxobutanoate + CO2 + NADH

-

Pseudomonas fluorescens

?

(R)-3,3-dimethylmalate + NAD+

Pseudomonas fluorescens UK-1

-

3-methyl-2-oxobutanoate + CO2 + NADH

-

Pseudomonas fluorescens UK-1

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

(R)-3,3-dimethylmalate + NAD+

-

207978

Pseudomonas fluorescens

3-methyl-2-oxobutanoate + CO2 + NADH

-

207978

Pseudomonas fluorescens

?

(R)-3,3-dimethylmalate + NAD+

-

207978

Pseudomonas fluorescens UK-1

3-methyl-2-oxobutanoate + CO2 + NADH

-

207978

Pseudomonas fluorescens UK-1

?

D-malate + NAD+

-

207978

Pseudomonas fluorescens

pyruvate + CO2 + NADH

-

207978

Pseudomonas fluorescens

?

D-malate + NAD+

-

207978

Pseudomonas fluorescens UK-1

pyruvate + CO2 + NADH

-

207978

Pseudomonas fluorescens UK-1

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

207978

Lähdesmäki

Comparison of D-malate and bet ...

Pseudomonas fluorescens

Biochim. Biophys. Acta

613

266-274

1980

-

-

-

-

-

-

2

3

-

-

2

2

-

4

-

-

-

-

-

-

1

-

4

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

2

-

3

-

-

2

2

-

-

-

-

-

-

1

-

4

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

207979

Mäntsälä

Purification of pantoate and d ...

Pseudomonas fluorescens

Biochim. Biophys. Acta

526

25-33

1978

-

-

-

-

-

-

-

-

-

-

3

2

-

4

-

-

1

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

3

2

-

-

-

1

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

207980

Magee

The bacterial degradation of p ...

Pseudomonas sp., Pseudomonas sp. P-2

Biochemistry

5

409-416

1966

-

-

-

-

-

-

-

4

-

4

-

2

-

2

-

-

1

-

-

-

1

1

6

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

4

-

4

-

2

-

-

-

1

-

-

1

1

6

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-