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Literature summary for 1.1.1.82 extracted from

  • Lemaire, M.; Miginiac-Maslow, M.; Decottignies, P.
    The catalytic site of chloroplastic NADP-dependent malate dehydrogenase contains a His/Asp pair (1996), Eur. J. Biochem., 236, 947-952.
    View publication on PubMed
Search for more literature for 1.1.1.82

Protein Variants

Protein Variants Comment Organism
D201A only slightly active, 80fold increased Km for oxaloacetate, Km-value for NADPH is slightly decreased Sorghum sp.
D201N only slightly active, 45fold increased Km for oxaloacetate, Km-value for NADPH is slightly decreased Sorghum sp.
H229N no activity Sorghum sp.
H229Q no activity Sorghum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0114
-
 NADPH mutant enzyme D201N Sorghum sp.
0.023
-
 NADPH mutant enzyme D201A Sorghum sp.
0.04
-
 oxaloacetate wild-type enzyme Sorghum sp.
0.0446
-
 NADPH wild-type enzyme Sorghum sp.
1.8
-
 oxaloacetate mutant enzyme D201N Sorghum sp.
3
-
 oxaloacetate mutant enzyme D201A Sorghum sp.
5
-
 (S)-malate mutant enzyme D201A Sorghum sp.
10.2
-
 (S)-malate mutant enzyme D201N Sorghum sp.
12
-
 (S)-malate wild-type enzyme Sorghum sp.

Organism

Organism UniProt Comment Textmining
Sorghum sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Sorghum sp. oxaloacetate + NADPH + H+
-
 r
oxaloacetate + NADPH
-
 Sorghum sp. (S)-malate + NADP+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 (S)-malate mutant enzyme D201A Sorghum sp.
0.9
-
 (S)-malate mutant enzyme D201N Sorghum sp.
110
-
 oxaloacetate mutant enzyme D201N Sorghum sp.
130
-
 oxaloacetate mutant enzyme D201A Sorghum sp.
233
-
 (S)-malate wild-type enzyme D201A Sorghum sp.
1520
-
 oxaloacetate wild-type enzyme Sorghum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Sorghum sp.