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Literature summary for 1.1.1.81 extracted from

  • Kutner, J.; Shabalin, I.G.; Matelska, D.; Handing, K.B.; Gasiorowska, O.; Sroka, P.; Gorna, M.W.; Ginalski, K.; Wozniak, K.; Minor, W.
    Structural, biochemical, and evolutionary characterizations of glyoxylate/hydroxypyruvate reductases show their division into two distinct subfamilies (2018), Biochemistry, 57, 963-977 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.81

Crystallization (Commentary)

Crystallization (Comment) Organism
structures in apo forms and in complexes with cofactors and substrates/substrate analogues Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0105
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
0.059
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
5.59
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
6.14
-
 3-hydroxypyruvate K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C Sinorhizobium meliloti
6.58
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
9.35
-
 3-hydroxypyruvate pH 7.5, 25°C Sinorhizobium meliloti
9.69
-
 pyruvate pH 7.5, 25°C Sinorhizobium meliloti
11
-
 2-dehydro-D-gluconate pH 7.5, 25°C Sinorhizobium meliloti
12.3
-
 glyoxylate K0.5 value, Hill coefficient 1.83, pH 7.5, 25°C Sinorhizobium meliloti
80
-
 glyoxylate K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti Q92LZ4
-
-
Sinorhizobium meliloti Q92T34
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gluconate + NADPH + H+
-
 Sinorhizobium meliloti D-gluconate + NADP+
-
 ?
3-hydroxypyruvate + NADPH + H+
-
 Sinorhizobium meliloti D-glycerate + NADP+
-
 ?
glyoxylate + NADPH + H+
-
 Sinorhizobium meliloti glycolate + NADP+
-
 ?
phenylpyruvate + NADPH + H+
-
 Sinorhizobium meliloti phenyllactate + NADP+
-
 ?
pyruvate + NADPH + H+
-
 Sinorhizobium meliloti lactate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
GhrA
-
 Sinorhizobium meliloti
GhrB
-
 Sinorhizobium meliloti
SMc02828
-
 Sinorhizobium meliloti
SMc04462
-
 Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Sinorhizobium meliloti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.057
-
 pyruvate pH 7.5, 25°C Sinorhizobium meliloti
0.27
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
0.37
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
0.81
-
 glyoxylate K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C Sinorhizobium meliloti
0.94
-
 2-dehydro-D-gluconate pH 7.5, 25°C Sinorhizobium meliloti
1.2
-
 3-hydroxypyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.72
-
 glyoxylate Hill coefficient 1.83, pH 7.5, 25°C Sinorhizobium meliloti
9.31
-
 3-hydroxypyruvate K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C Sinorhizobium meliloti
371
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
773
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti