Literature summary for 1.1.1.81 extracted from

Sugimoto, E.; Kitagawa, Y.; Nakanishi, K.; Chiba, H.
Purification and properties of beef liver D-glycerate dehydrogenase (1972), J. Biochem., 72, 1307-1315.

Search for more literature for 1.1.1.81

General Stability

General Stability	Organism
dithiothreitol stabilizes	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-diphospho-D-glycerate	1 mM, 83% and 91% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus
2-phospho-DL-glycerate	1 mM, 93% and 81% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus
3-phospho-D-glycerate	1 mM, 53% and 65% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus
Ag+	0.1 mM, complete inhibition	Bos taurus
alpha-D-fructose 1,6-diphosphate	0.1 mM, 28% and 74% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus
ATP	1 mM, 62% and 64% inhibition of liver and spinal cord enzyme respectively, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus
Hg2+	0.1 mM, complete inhibition	Bos taurus
p-chloromercuribenzoate	0.1 mM, complete inhibition	Bos taurus
pyruvate	1 mM, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	Hydroxypyruvate	-	Bos taurus
0.037	-	NADP+	-	Bos taurus
0.077	-	NAD+	-	Bos taurus
1.4	-	DL-glycerate	-	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
72000	-	sedimentation velocity analysis	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hydroxypyruvate + NAD(P)H	Bos taurus	-	D-glycerate + NAD(P)+ + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.67	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-glycerate + NAD+	-	Bos taurus	3-hydroxypyruvate + NADH + H+	-	r
hydroxypyruvate + NAD(P)H	-	Bos taurus	D-glycerate + NAD(P)+ + H+	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	hydroxypyruvate reduction	Bos taurus
9.3	-	D-glycerate oxidation	Bos taurus

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Release 2023.1 (January 2023)