Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | - |
Urocitellus richardsonii | |
guanidinium hydrochloride | - |
Urocitellus richardsonii | |
Urea | - |
Urocitellus richardsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview. Relative to euthermic liver G3PDH, hibernator liver G3PDH has a decreased affinity for its substrate, glycerol-3-phosphate, at 37°C and 22°C, while at 5°C, there is a significant increase in the affinity for G3P in the hibernating form of the enzyme, relative to the euthermic form. A change in pH from pH 8.0 to pH 7.5 leads to increased G3PDH affinity for NAD+. With a change in pH from pH 8.0 to pH 7.5 at 22°C in the euthermic and hibernator conditions, the Km for NAD+ decreases by 62% and 53%, respectively | Urocitellus richardsonii | |
0.09 | - |
NAD+ | pH 8.0, 5°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.12 | - |
NAD+ | pH 8.0, 5°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.2 | - |
NAD+ | enzyme from euthermic animal, at pH 7.5 and 22°C | Urocitellus richardsonii | |
0.2 | - |
NAD+ | enzyme from hibernating animal, at pH 7.5 and 22°C | Urocitellus richardsonii | |
0.2 | - |
NAD+ | pH 7.5, 22°C, euthermic G3PDH enzyme and hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.32 | - |
NAD+ | enzyme from hibernating animal, at pH 8.0 and 37°C | Urocitellus richardsonii | |
0.32 | - |
NAD+ | pH 8.0, 37°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.39 | - |
NAD+ | enzyme from euthermic animal, at pH 8.0 and 37°C | Urocitellus richardsonii | |
0.39 | - |
NAD+ | pH 8.0, 37°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.41 | - |
sn-glycerol 3-phosphate | enzyme from hibernating animal, at pH 7.5 and 22°C | Urocitellus richardsonii | |
0.41 | - |
sn-glycerol 3-phosphate | pH 7.5, 22°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.42 | - |
NAD+ | enzyme from hibernating animal, at pH 8.0 and 22°C | Urocitellus richardsonii | |
0.42 | - |
NAD+ | pH 8.0, 22°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.48 | - |
sn-glycerol 3-phosphate | enzyme from hibernating animal, at pH 8.0 and 5°C | Urocitellus richardsonii | |
0.48 | - |
sn-glycerol 3-phosphate | pH 8.0, 5°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.5 | - |
NAD+ | enzyme from euthermic animal, at pH 7.5 and 37°C | Urocitellus richardsonii | |
0.5 | - |
NAD+ | pH 7.5, 37°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.52 | - |
NAD+ | enzyme from euthermic animal, at pH 8.0 and 22°C | Urocitellus richardsonii | |
0.52 | - |
NAD+ | pH 8.0, 22°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.57 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 8.0 and 22°C | Urocitellus richardsonii | |
0.57 | - |
sn-glycerol 3-phosphate | pH 8.0, 22°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.61 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 7.5 and 22°C | Urocitellus richardsonii | |
0.61 | - |
sn-glycerol 3-phosphate | pH 7.5, 22°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.63 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 7.5 and 37°C | Urocitellus richardsonii | |
0.63 | - |
sn-glycerol 3-phosphate | pH 7.5, 37°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.69 | - |
NAD+ | enzyme from hibernating animal, at pH 7.5 and 37°C | Urocitellus richardsonii | |
0.69 | - |
NAD+ | pH 7.5, 37°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
0.83 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 8.0 and 5°C | Urocitellus richardsonii | |
0.83 | - |
sn-glycerol 3-phosphate | pH 8.0, 5°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
0.88 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 8.0 and 37°C | Urocitellus richardsonii | |
0.88 | - |
sn-glycerol 3-phosphate | pH 8.0, 37°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
1.17 | - |
sn-glycerol 3-phosphate | enzyme from hibernating animal, at pH 7.5 and 37°C | Urocitellus richardsonii | |
1.17 | - |
sn-glycerol 3-phosphate | pH 7.5, 37°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
1.51 | - |
sn-glycerol 3-phosphate | enzyme from euthermic animal, at pH 7.5 and 5°C | Urocitellus richardsonii | |
1.51 | - |
sn-glycerol 3-phosphate | pH 7.5, 5°C, euthermic G3PDH enzyme | Urocitellus richardsonii | |
1.56 | - |
sn-glycerol 3-phosphate | enzyme from hibernating animal, at pH 8.0 and 37°C | Urocitellus richardsonii | |
1.56 | - |
sn-glycerol 3-phosphate | pH 8.0, 37°C, hibernation G3PDH enzyme | Urocitellus richardsonii | |
1.61 | - |
sn-glycerol 3-phosphate | enzyme from hibernating animal, at pH 8.0 and 22°C | Urocitellus richardsonii | |
1.61 | - |
sn-glycerol 3-phosphate | pH 8.0, 22°C, hibernation G3PDH enzyme | Urocitellus richardsonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Urocitellus richardsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Urocitellus richardsonii | relative to euthermic liver G3PDH, hibernator liver G3PDH has a decreased affinity for its substrate, glycerol-3-phosphate (G3P) at 37°C and 22°C, while at 5°C, there is a significant increase in affinity for G3P in the hibernating form of the enzyme, relative to the euthermic form | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Urocitellus richardsonii | - |
glycerone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Urocitellus richardsonii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | potential phosphorylation site Tyr83 is responsible for the differential phosphorylation between euthermic and hibernator G3PDH. There is a 1.70fold increase in phosphorylation of hibernating G3PDH relative to euthermic G3PDH | Urocitellus richardsonii |
Purification (Comment) | Organism |
---|---|
hydroxyapatite column chromatography, CM-column chromatography, and Cibacron blue affinity column chromatography | Urocitellus richardsonii |
native enzyme from liver cell extract of euthermic and hibernating animals 272.5-273.5fold by hydroxyapatite chromatography, buffer exchange gel filtration, cation exchange chromatography, affinity chromatography, and ultrafiltration | Urocitellus richardsonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Urocitellus richardsonii | - |
liver | from eurthermic and hibernating animals | Urocitellus richardsonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.174 | - |
purified euthermic G3PDH enzyme, pH 8.0, 22°C | Urocitellus richardsonii |
2.9 | - |
purified hibernator G3PDH enzyme, pH 8.0, 22°C | Urocitellus richardsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NADH + H+ | - |
Urocitellus richardsonii | sn-glycerol 3-phosphate + NAD+ | - |
r | |
additional information | relative to euthermic liver G3PDH, hibernator liver G3PDH has a decreased affinity for its substrate, glycerol-3-phosphate (G3P) at 37°C and 22°C, while at 5°C, there is a significant increase in affinity for G3P in the hibernating form of the enzyme, relative to the euthermic form | Urocitellus richardsonii | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Urocitellus richardsonii | glycerone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Urocitellus richardsonii |
More | three-dimensional structure modeling of enzyme G3PDH to identify the potential phosphorylation site Tyr83 responsible for the differential phosphorylation between euthermic and hibernator G3PDH, predicted tertiary and quaternary structures, overview | Urocitellus richardsonii |
Synonyms | Comment | Organism |
---|---|---|
G3PDH | - |
Urocitellus richardsonii |
glycerol 3-phosphate dehydrogenase | - |
Urocitellus richardsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 37 | assay at, glycerol 3-phosphate oxidation | Urocitellus richardsonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the half-life at 50°C is 6.74 min in euthermic and 14.31 min in hibernating animals | Urocitellus richardsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at, glycerol 3-phosphate oxidation | Urocitellus richardsonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
A change in pH from pH 8.0 to pH 7.5 leads to increased G3PDH affinity for NAD+. With a change in pH from pH 8.0 to pH 7.5 at 22°C in the euthermic and hibernator conditions, the Km for NAD+ decreases by 62% and 53%, respectively | Urocitellus richardsonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Urocitellus richardsonii | |
NADH | - |
Urocitellus richardsonii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.57 | - |
enzyme from hibernating animal, at pH 7.5 and 22°C | Urocitellus richardsonii | guanidine hydrochloride | |
0.57 | - |
pH 8.0, 22°C, hibernation G3PDH enzyme | Urocitellus richardsonii | guanidinium hydrochloride | |
0.69 | - |
enzyme from euthermic animal, at pH 7.5 and 22°C | Urocitellus richardsonii | guanidine hydrochloride | |
0.69 | - |
pH 8.0, 22°C, euthermic G3PDH enzyme | Urocitellus richardsonii | guanidinium hydrochloride | |
1.8 | - |
enzyme from hibernating animal, at pH 7.5 and 22°C | Urocitellus richardsonii | Urea | |
1.8 | - |
pH 8.0, 22°C, hibernation G3PDH enzyme | Urocitellus richardsonii | Urea | |
2.22 | - |
enzyme from euthermic animal, at pH 7.5 and 22°C | Urocitellus richardsonii | Urea | |
2.22 | - |
pH 8.0, 22°C, euthermic G3PDH enzyme | Urocitellus richardsonii | Urea |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional structure modeling of enzyme G3PDH to identify the potential phosphorylation site (83Tyr) responsible for the differential phosphorylation between euthermic and hibernator G3PDH. Structural and functional changes in G3PDH support the enzyme's function at a low core body temperature experienced during the species hibernating season | Urocitellus richardsonii |
physiological function | the hibernating form of G3PDH from the liver of Richardson's ground squirrels maintains better functionality at low temperatures and has greater temperature stability. These properties help sustain G3PDH function over the wide range of body temperatures experienced by the species as they cycle though torpor-arousal bouts over the hibernating season | Urocitellus richardsonii |