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Literature summary for 1.1.1.8 extracted from

  • Zolnierowicz, S.; Swierczynski, J.; Zelewski, L.
    Isolation and properties of glycerol-3-phosphate oxidoreductase from human placenta (1986), Eur. J. Biochem., 154, 161-166.
    View publication on PubMed

General Stability

General Stability Organism
ammonium sulfate inactivates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
NADH measured at pH 7.5 Homo sapiens
0.026
-
dihydroxyacetone phosphate measured at pH 7.5 Homo sapiens
0.036
-
NAD+ measured at pH 9.0 Homo sapiens
0.143
-
sn-glycerol-3-phosphate measured at pH 9.0 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Homo sapiens
62000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Homo sapiens
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88.2
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Homo sapiens dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.7 8.7 about 80% of maximal activity at pH 6.7 and 8.7, reduction of dihydroxyacetone phosphate, decrease of activity below and above these pH values Homo sapiens
8.1 9.4 about 80% of maximal activity at pH 8.1 and 9.4, oxidation of glycerol-3-phosphate, decrease of activity below and rapidly above these pH values Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens