Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.79 extracted from

  • Zhang, Z.; Liang, X.; Lu, L.; Xu, Z.; Huang, J.; He, H.; Peng, X.
    Two glyoxylate reductase isoforms are functionally redundant but required under high photorespiration conditions in rice (2020), BMC Plant Biol., 20, 357 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.79

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Oryza sativa Japonica Group

Inhibitors

Inhibitors Comment Organism Structure
oxalate when using NADPH as cofactor, the Ki value of oxalate for isoform GR1 is 21.2 mM and that for isoform GR2 is 290.8 mM. When using NADH as cofactor, the Ki values of oxalate are much lower, 3.6mM for isoform GR1, and 8.2mM for isoform GR2 Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0176
-
 NADPH isoform GR1, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.0304
-
 glyoxylate isoform GR1, with NADPH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.053
-
 NADPH isoform GR2, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.0721
-
 glyoxylate isoform GR2, with NADPH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.1446
-
 glyoxylate isoform GR2, with NADH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.2677
-
 glyoxylate isoform GR1, with NADH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.4039
-
 NADH isoform GR2, at pH 7.4 and 30°C Oryza sativa Japonica Group
0.4202
-
 NADH isoform GR1, at pH 7.4 and 30°C Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isoform GR1 Oryza sativa Japonica Group 9507
-
cytosol isoform GR1 Oryza sativa Japonica Group 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + NADP+ Oryza sativa Japonica Group
-
 glyoxylate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group
-
 cultivar Zhonghua 11
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity Ni-IDA resin column chromatography Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
glume
-
 Oryza sativa Japonica Group
-
leaf
-
 Oryza sativa Japonica Group
-
leaf sheath
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + NADP+
-
 Oryza sativa Japonica Group glyoxylate + NADPH + H+
-
 ?
glyoxylate + NADH + H+ NADPH-dependent activity is much higher than the NADH-dependent activity Oryza sativa Japonica Group glycolate + NAD+
-
 ?
glyoxylate + NADPH + H+ NADPH-dependent activity is much higher than the NADH-dependent activity Oryza sativa Japonica Group glycolate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, isoform GR1, SDS-PAGE Oryza sativa Japonica Group
? x * 37000, isoform GR2, SDS-PAGE Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
GR1 isoform Oryza sativa Japonica Group
GR2 isoform Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
NADPH the enzyme prefers NADPH to NADH as cofactor Oryza sativa Japonica Group

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6
-
 oxalate isoform GR1, with glyoxylate and NADH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
8.2
-
 oxalate isoform GR2, with glyoxylate and NADH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
212
-
 oxalate isoform GR1, with glyoxylate and NADPH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
290.8
-
 oxalate isoform GR2, with glyoxylate and NADPH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group

General Information

General Information Comment Organism
physiological function isoforms GR1 and GR2 function redundantly in detoxifying glyoxylate in rice plants under normal growth conditions, whereas both are simultaneously required under high photorespiration conditions Oryza sativa Japonica Group