Cloned (Comment) | Organism |
---|---|
ORF YNL274c, DNA and amino acid sequence determination and analysis, expression in yeast strains, overview | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADPH + H+ | Saccharomyces cerevisiae | - |
glycolate + NADP+ | - |
r | |
glyoxylate + NADPH + H+ | Saccharomyces cerevisiae BY4742 | - |
glycolate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
ORF YNL274c | - |
Saccharomyces cerevisiae BY4742 | - |
ORF YNL274c | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of wild-type and mutant strains, overview | Saccharomyces cerevisiae |
0.04 | - |
strain BY4742, substrate glyoxylate | Saccharomyces cerevisiae |
13.8 | - |
purified recombinant enzyme, substrate glyoxylate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisocaproate + NADPH + H+ | low activity | Saccharomyces cerevisiae | 2-hydroxy-4-methylpentanoate + NADP+ | - |
? | |
2-oxoisocaproate + NADPH + H+ | low activity | Saccharomyces cerevisiae BY4742 | 2-hydroxy-4-methylpentanoate + NADP+ | - |
? | |
glyoxylate + NADPH + H+ | - |
Saccharomyces cerevisiae | glycolate + NADP+ | - |
r | |
glyoxylate + NADPH + H+ | - |
Saccharomyces cerevisiae BY4742 | glycolate + NADP+ | - |
r | |
hydroxypyruvate + NADPH + H+ | 15% activity compared to glyoxylate | Saccharomyces cerevisiae | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | 15% activity compared to glyoxylate | Saccharomyces cerevisiae BY4742 | D-glycerate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GOR1 | - |
Saccharomyces cerevisiae |
More | cf. EC 1.1.1.26 | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae | |
NADPH | preferred cofactor, NADH gives only 4% of the activity with NADPH | Saccharomyces cerevisiae |