Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybutyrate | mixed type inhibition with NADPH; mixed type inhibition with succinic semialdehyde | Arabidopsis thaliana | |
glycolate | mixed type inhibition with glyoxylate; uncompetitive with NADPH | Arabidopsis thaliana | |
NADP+ | competitive with NADPH; competitive with NADPH when glyoxylate is the fixed substrate; uncompetitive with glyoxylate; uncompetitive with succinic semialdehyde | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
glyoxylate | recombinant protein from Escherichia coli | Arabidopsis thaliana | |
0.87 | - |
Succinic semialdehyde | recombinant protein from Escherichia coli | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LSV0 | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency; L. Heynh | - |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glycolate + NADP+ = glyoxylate + NADPH + H+ | ordered Bi Bi mechanism, complexation of NADPH to the enzyme before glyoxylate or succinic semialdehyde, and release of NADP+ before glycolate or 4-hydroxybutyrate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADPH + H+ | preferred substrate | Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
succinic semialdehyde + NADPH + H+ | - |
Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
AtGR1 | - |
Arabidopsis thaliana |
More | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
NADP+ | competitive with NADPH | Arabidopsis thaliana | |
0.0095 | - |
NADP+ | competitive with NADPH when glyoxylate is the fixed substrate | Arabidopsis thaliana | |
0.125 | - |
NADP+ | uncompetitive with succinic semialdehyde | Arabidopsis thaliana | |
0.147 | - |
NADP+ | uncompetitive with glyoxylate | Arabidopsis thaliana | |
22.1 | - |
glycolate | uncompetitive with NADPH | Arabidopsis thaliana | |
23.7 | - |
glycolate | mixed type inhibition with glyoxylate | Arabidopsis thaliana | |
79.9 | - |
4-hydroxybutyrate | mixed type inhibition with NADPH | Arabidopsis thaliana | |
93.5 | - |
4-hydroxybutyrate | mixed type inhibition with succinic semialdehyde | Arabidopsis thaliana |