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Literature summary for 1.1.1.79 extracted from

  • Yokota, A.; Haga, S.; Kitaoka, S.
    Purification and some properties of glyoxylate reductase (NADP+) and its functional location in mitochondria in Euglena gracilis Z (1985), Biochem. J., 227, 211-216.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.79

Inhibitors

Inhibitors Comment Organism Structure
adenine 0.01 mM, 70% inhibition after preincubation for 5 min Euglena gracilis
ATP 10 mM, 20% inhibition Euglena gracilis
N-ethylmaleimide 10.0 mM, 75% inhibition after preincubation for 5 min Euglena gracilis
p-chloromercuribenzoate 0.1 mM, 55% inhibition after preincubation for 5 min Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 NADPH
-
 Euglena gracilis
0.045
-
 glyoxylate
-
 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion intermembrane space and inner membrane Euglena gracilis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NAD(P)H Euglena gracilis specific for NADPH glycolate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ethanol precipitation, DEAE-cellulose, CM-cellulose, affinity chromatography Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.1
-
-
 Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + H+ 13.9% of glyoxylate activity Euglena gracilis 2-hydroxyglutarate + NAD+
-
 ?
acetaldehyde + NADPH + H+ 10.4% of glyoxylate activity Euglena gracilis ethanol + NADP+
-
 ?
glyoxylate + NAD(P)H specific for NADPH Euglena gracilis glycolate + NAD(P)+
-
 ?
oxaloacetate + NADPH 28.6% of glyoxylate activity Euglena gracilis malate + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.45
-
 enzyme activity decreases by 40% and 50% at pH 5.5 and 8.0 respectively Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
NAD(P)H specific for NADPH Euglena gracilis
NADPH specific for NADPH Euglena gracilis