Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Ba2+, Ca2+, Mn2+, Mg2+ and Co2+ activate enzyme 1.6-1.8 fold at a concentration of 5 mM | Corynebacterium glutamicum |
Application | Comment | Organism |
---|---|---|
synthesis | preparation of chiral acetoinic compounds, enzymic identification for chiral acetoinic compounds or as model enzyme for studying the interrelation between enzymic stereospecificity and structure | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109/pLBD2-CTC | Corynebacterium glutamicum |
L-butanediol gene containing plasmid pLBD2-CTC is expressed in Escherichia coli JM109 | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | competitive inhibition; competitive inhibitor | Corynebacterium glutamicum | |
acetate | slight inhibition at 5 mM | Corynebacterium glutamicum | |
Cu2+ | 5 mM, 89% inhibition; 89% inhibition at 5 mM | Corynebacterium glutamicum | |
formate | slight inhibition at 5 mM | Corynebacterium glutamicum | |
Hg2+ | 100% inhibition at 5 mM; 5 mM, 100% inhibition | Corynebacterium glutamicum | |
Lactate | slight inhibition at 5 mM | Corynebacterium glutamicum | |
additional information | slight inhibition of 10-20% by organic acids at concentrations of 5 mM: lactate, pyruvate, succinate, acetate and formate | Corynebacterium glutamicum | |
pyruvate | slight inhibition at 5 mM | Corynebacterium glutamicum | |
succinate | slight inhibition at 5 mM | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | pH 6.0 | Corynebacterium glutamicum | |
0.07 | - |
NAD+ | pH 10.5 | Corynebacterium glutamicum | |
0.1 | - |
NADH | pH 6.0 | Corynebacterium glutamicum | |
0.22 | - |
(S,S)-butane-2,3-diol | pH 6.0 | Corynebacterium glutamicum | |
0.22 | - |
(S,S)-butane-2,3-diol | pH 10.5 | Corynebacterium glutamicum | |
0.44 | - |
L-acetoin | pH 6.0 | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Corynebacterium glutamicum | |
Ba2+ | Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM | Corynebacterium glutamicum | |
Ca2+ | - |
Corynebacterium glutamicum | |
Ca2+ | Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM | Corynebacterium glutamicum | |
Co2+ | - |
Corynebacterium glutamicum | |
Co2+ | Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM | Corynebacterium glutamicum | |
Mg2+ | - |
Corynebacterium glutamicum | |
Mg2+ | Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM | Corynebacterium glutamicum | |
Mn2+ | - |
Corynebacterium glutamicum | |
Mn2+ | Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM | Corynebacterium glutamicum | |
additional information | no significant changes in enzyme activity with 5 mM Ni2+, Zn2+ and Cd2+ | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27107 | - |
4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence | Corynebacterium glutamicum |
30000 | - |
4 * 30000, SDS-PAGE | Corynebacterium glutamicum |
30000 | - |
4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence | Corynebacterium glutamicum |
110000 | - |
gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
C-1012 | - |
Corynebacterium glutamicum C-1012 | - |
C-1012 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Corynebacterium glutamicum |
streptomycin sulfate precipitation, affinity chromatography, gel filtration | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Corynebacterium glutamicum |
277.6 | - |
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-pentanedione + NADH + H+ | 7% activity in comparison to L-acetoin | Corynebacterium glutamicum | ? | - |
r | |
2,3-pentanedione + NADH + H+ | 7% activity in comparison to L-acetoin | Corynebacterium glutamicum C-1012 | ? | - |
r | |
diacetyl + NADH | also reduction of 2,3-pentanedione | Corynebacterium glutamicum | L-acetoin + NAD+ | - |
? | |
diacetyl + NADH | also reduction of 2,3-pentanedione | Corynebacterium glutamicum C-1012 | L-acetoin + NAD+ | - |
? | |
diacetyl + NADH + H+ | 35% activity in comparison to L-acetoin | Corynebacterium glutamicum | ? | - |
r | |
L-acetoin + NADH | short chain dehydrogenase reductase family | Corynebacterium glutamicum | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | no oxidadion of several alcohols | Corynebacterium glutamicum | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | short chain dehydrogenase reductase family | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | no oxidadion of several alcohols | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
L-acetoin + NADH + H+ | 100% activity | Corynebacterium glutamicum | (S,S)-butane-2,3-diol + NAD+ | - |
r | |
additional information | not: meso-butanediol, D-butanediol, 2-butanol, 1,2-propanediol, ethanol, acetol, 1,2-butanediol, 1,3-butanediol, n-butanol, n-propanol, D-acetoin, acetol, dihydroxyacetone, 2,4-pentanedione | Corynebacterium glutamicum | ? | - |
? | |
additional information | not: meso-butanediol, D-butanediol, 2-butanol, 1,2-propanediol, ethanol, acetol, 1,2-butanediol, 1,3-butanediol, n-butanol, n-propanol, D-acetoin, acetol, dihydroxyacetone, 2,4-pentanedione | Corynebacterium glutamicum C-1012 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE | Corynebacterium glutamicum |
tetramer | 4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence | Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
up to, rapidly inactivated over 40°C, 30 min incubation before the enzyme assay | Corynebacterium glutamicum |
37 | 40 | The enzyme is stable up to 37°C but rapidly inactivated over 40°C | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of L-acetoin | Corynebacterium glutamicum |
10.5 | - |
oxidation of L-butanediol | Corynebacterium glutamicum |
10.5 | - |
oxidation of (S,S)-butane-2,3-diol | Corynebacterium glutamicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Corynebacterium glutamicum |
7 | 9 | stable | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum | |
NADH | - |
Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.28 | - |
2-mercaptoethanol | Dixon plot | Corynebacterium glutamicum | |
4.28 | - |
2-mercaptoethanol | pH 6.0 | Corynebacterium glutamicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corynebacterium glutamicum | isoelectric focussing | - |
4.9 |