Application | Comment | Organism |
---|---|---|
analysis | enzymatic quantification of 5-keto-D-gluconate | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3), expression levels vary from colony to colony, cells showing high enzyme expression tend to show less growth, expression system appears to be unstable as expression level decreases when subcultured for several times, best expression results with freshly transformed cells | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gluconobacter oxydans | production of 5-keto D-gluconate from D-gluconate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
NBRC3172, also Gluconobacter suboxydans IFO12528, genetically identical with Gluconobacter oxydans 621H | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
4°C, 10 mM phosphate buffer, pH 6.0, no added 2-mercaptoethanol, fully stable for at least 3 days | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-keto D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
? | |
additional information | production of 5-keto D-gluconate from D-gluconate | Gluconobacter oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-keto D-gluconate reductase | - |
Gluconobacter oxydans |
5KGA reductase | - |
Gluconobacter oxydans |
5KGR | - |
Gluconobacter oxydans |
GOX2187 gene product | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gluconobacter oxydans |