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Literature summary for 1.1.1.69 extracted from

  • Salusjarvi, T.; Povelainen, M.; Hvorslev, N.; Eneyskaya, E.V.; Kulminskaya, A.A.; Shabalin, K.A.; Neustroev, K.N.; Kalkkinen, N.; Miasnikov, A.N.
    Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia coli strain (2004), Appl. Microbiol. Biotechnol., 65, 306-314.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
D-arabitol pH 5.5, 30°C Gluconobacter oxydans
57
-
D-sorbitol pH 5.5, 30°C Gluconobacter oxydans
161
-
D-gluconic acid pH 5.5, 30°C Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Gluconobacter oxydans 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q70JN9 large subunit; recombinant enzyme
-
Gluconobacter oxydans Q70JP0 small subunit; recombinant enzyme
-
Gluconobacter oxydans IFO 12528 Q70JN9 large subunit; recombinant enzyme
-
Gluconobacter oxydans IFO 12528 Q70JP0 small subunit; recombinant enzyme
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
pH 5.5, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanediol + NAD+ 169% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
2,3-butanediol + NAD+ 169% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
?
D-arabitol + NAD+ 130% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
D-arabitol + NAD+ 130% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
?
D-gluconic acid + NAD+ 8% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
D-gluconic acid + NAD+ 8% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
?
D-mannitol + NAD+ 74% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
D-mannitol + NAD+ 74% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
?
D-sorbitol + NAD+
-
Gluconobacter oxydans ? + NADH
-
?
D-sorbitol + NAD+
-
Gluconobacter oxydans IFO 12528 ? + NADH
-
?
glycerol + NAD+ 138% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
meso-erytritol + NAD+ 143% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?
ribitol + NAD+ 66% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
fairly stable Gluconobacter oxydans
8
-
unstable Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Gluconobacter oxydans
NADH
-
Gluconobacter oxydans