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Literature summary for 1.1.1.67 extracted from
- Characterization of a thermostable mannitol dehydrogenase from hyperthermophilic Thermotoga neapolitana DSM 4359 with potential application in mannitol production (2016), J. Mol. Catal. B, 134, 122-128.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Thermotoga neapolitana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | about 20% residual activity at 1 mM | Thermotoga neapolitana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | about 300% activity at 1 mM | Thermotoga neapolitana | |
| Ca2+ | about 220% activity at 1 mM | Thermotoga neapolitana | |
| Co2+ | about 400% activity at 1 mM | Thermotoga neapolitana | |
| Mg2+ | about 150% activity at 1 mM | Thermotoga neapolitana | |
| Ni2+ | about 230% activity at 1 mM | Thermotoga neapolitana | |
| Zn2+ | about 110% activity at 1 mM | Thermotoga neapolitana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
4 * 36000, SDS-PAGE | Thermotoga neapolitana |
| 135000 | - |
gel filtration | Thermotoga neapolitana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | Thermotoga neapolitana | - |
D-mannitol + NAD+ | - |
? | |
| D-fructose + NADH + H+ | Thermotoga neapolitana DSM 4359 | - |
D-mannitol + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
- |
- |
| Thermotoga neapolitana DSM 4359 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | - |
Thermotoga neapolitana | D-mannitol + NAD+ | - |
? | |
| D-fructose + NADH + H+ | - |
Thermotoga neapolitana DSM 4359 | D-mannitol + NAD+ | - |
? | |
| additional information | the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors | Thermotoga neapolitana | ? | - |
? | |
| additional information | the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors | Thermotoga neapolitana DSM 4359 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 36000, SDS-PAGE | Thermotoga neapolitana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MtDH | - |
Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga neapolitana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 95 | more than 60% of the maximal activity between 80 and 95°C | Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 90 | the enzyme records 85%, 50% and 10% of its initial activity after 4 h of exposure to 75, 80, 85 and 90°C, respectively. The enzyme almost does not lose the activity after 1 h of incubation at 75°C and still has half of initial activity after incubated at 90°C for 1 h | Thermotoga neapolitana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 180 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermotoga neapolitana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | more than 60% of the maximal activity in the pH range 6.09.0 | Thermotoga neapolitana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermotoga neapolitana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana | |
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