Literature summary for 1.1.1.67 extracted from

Klimacek, M.; Brunsteiner, M.; Nidetzky, B.
Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens: mobile GLU292 controls proton relay through a water channel that connects the active site with bulk solvent (2012), J. Biol. Chem., 287, 6655-6667.

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Protein Variants

Protein Variants	Comment	Organism
E292A	mutation partially disrupts the catalytic cycle. Role for residue Glu292 as a gate in a water chain mechanism of proton translocation. Removal of gatekeeper control in the E292A mutant results in a selective, up to 120fold slowing down of microscopicsteps immediately preceding catalytic oxidation of mannitol, consistent with the notion that formation of the productive enzyme-NAD-mannitol complex is promoted by a corresponding position change of Glu292	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	O08355	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose + NADH + H+	-	Pseudomonas fluorescens	D-mannitol + NAD+	-	r
D-mannitol + NAD+	-	Pseudomonas fluorescens	D-fructose + NADH + H+	-	r

Synonyms

Synonyms	Comment	Organism
MtlD	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)