Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme expressed in Bacillus megaterium is useful in production of D-mannitol using a resting cell biotransformation approach | Leuconostoc pseudomesenteroides |
Cloned (Comment) | Organism |
---|---|
gene mdh, high level expression in Bacillus megaterium requiring the adaptation of the corresponding ribosome binding site, the fdh gene is adapted to Bacillus megaterium codon usage via complete chemical gene synthesis, overview | Leuconostoc pseudomesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | D-mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol dehydrogenase gene from Leuconostoc pseudomesenteroides and the formate dehydrogenase gene, gene fdh from Mycobacterium vaccae N10, expression in Bacillus megaterium, development of an in vivo system, overview | Leuconostoc pseudomesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | Leuconostoc pseudomesenteroides | -mannitol production | D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | Leuconostoc pseudomesenteroides ATCC 12291 | -mannitol production | D-mannitol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc pseudomesenteroides | Q83VI5 | strain ATCC12291, gene mdh | - |
Leuconostoc pseudomesenteroides ATCC 12291 | Q83VI5 | strain ATCC12291, gene mdh | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | - |
Leuconostoc pseudomesenteroides | D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | -mannitol production | Leuconostoc pseudomesenteroides | D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | - |
Leuconostoc pseudomesenteroides ATCC 12291 | D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | -mannitol production | Leuconostoc pseudomesenteroides ATCC 12291 | D-mannitol + NAD+ | - |
r |