Literature summary for 1.1.1.62 extracted from

Ye, X.; Wang, H.; Kan, J.; Li, J.; Huang, T.; Xiong, G.; Hu, Z.
A novel 17beta-hydroxysteroid dehydrogenase in Rhodococcus sp. P14 for transforming 17beta-estradiol to estrone (2017), Chem. Biol. Interact., 276, 105-112 .

Search for more literature for 1.1.1.62

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21	Rhodococcus sp. P14

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0189	-	17beta-estradiol	pH 7.0, 25°C	Rhodococcus sp. P14

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus sp. P14	A0A1Z3FWF0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodococcus sp. P14

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
17beta-estradiol + NAD+	-	Rhodococcus sp. P14	estrone + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
17beta-HSD	-	Rhodococcus sp. P14
17beta-hydroxysteroid dehydrogenase	-	Rhodococcus sp. P14

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.38	-	17beta-estradiol	pH 7.0, 25°C	Rhodococcus sp. P14

Expression

Organism	Comment	Expression
Rhodococcus sp. P14	induced by estradiol	up

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
496	-	17beta-estradiol	pH 7.0, 25°C	Rhodococcus sp. P14

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Release 2023.1 (January 2023)